Staphylococcus Aureus ma le Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻaaogaina mo le suʻesuʻeina lelei o le staphylococcus aureus ma le methicillin-resistant staphylococcus aureus nucleic acids i faʻataʻitaʻiga o sputum tagata, faʻataʻitaʻiga o le nasal swab ma faʻamaʻi pipisi o le paʻu ma aano vaivai ile vitro.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-OT062 Staphylococcus Aureus ma le Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)

Tusi Faamaonia

CE

Epidemiology

Staphylococcus aureus o se tasi o siama faʻamaʻi taua o faʻamaʻi pipisi o le nosocomial.Staphylococcus aureus (SA) e a'afia i le staphylococcus ma o se sui o siama Gram-positive, lea e mafai ona maua ai le tele o vaila'au oona ma enzymes osofa'i.Ole siama ei ai uiga ole tufatufa lautele, malosi faʻamaʻi ma le maualuga o le tetee.Thermotable nuclease gene (nuc) o se kenera e sili ona fa'asaoina ole staphylococcus aureus.

Auala

FAM gene mecA e tetee i le methicillin
ROX

Pulea i totonu

CY5 staphylococcus aureus nuc gene

Vaega Fa'atekinisi

Teuina ≤-18℃ & puipuia mai le malamalama
Fa'asa-ola 12 masina
Ituaiga Fa'ata'ita'iga sputum, pa'u ma aano vaivai fa'ata'ita'iga fa'ama'i fa'ama'i, ma fa'ata'ita'iga o su'ega isu
Ct ≤36
CV ≤5.0%
LoD 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL siama e tetee i le methicillin.A iloa e le pusa le LoD fa'asinomaga a le atunu'u, 1000/mL staphylococcus aureus e mafai ona iloa.
Fa'apitoa O le su'ega fa'afeagai e fa'aalia ai o lenei pusa e leai se fa'afeagai fa'afeagai ma isi fa'ama'i manava e pei o le methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiumaniella, pneumonia. mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae.
Meafaigaluega Faatatau Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni

QuantStudio®5 Faiga PCR Taimi Moni

SLAN-96P Faiga PCR Taimi Moni

LightCycler®480 Taimi moni PCR faiga

LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni

MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela

BioRad CFX96 Taimi Moni PCR System

BioRad CFX Opus 96 Taimi moni PCR System

Galulue Galuega

Filifiliga 1.

Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e mafai ona fa'aogaina ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Fa'aopoopo le 200µL o le masima masani i le fa'agasologa fa'agaioiina, ma o la'asaga mulimuli ane e tatau ona toso mai e tusa ai ma fa'atonuga, ma o le voluma elution fautuaina o le 80μL.

Filifiliga 2.

Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Faaopopo le 1mL o le masima masani i le paʻu pe a uma ona fufulu ile saline masani, ona faʻafefiloi lelei.Centrifuge i le 13,000r/min mo le 5 minute, aveese le supernatant (fa'aagaga 10-20μL o le supernatant), ma mulimuli i fa'atonuga mo le su'eina mulimuli ane.

Fautuaina le fa'aputuina o mea fa'aputu: Nucleic Acid Extraction po'o le Purification Reagent (YDP302) e Tiangen Biotech (Beijing) Co.,Ltd.E tatau ona fa'atinoina le fa'atosina e tusa ai ma le Laasaga 2 o le tusi lesona.E fautuaina le fa'aogaina o le RNase ma le DNase-leai se vai mo le fa'afefe ma le voluma o le 100μL.

 


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