Adenovirus lautele
Igoa o oloa
HWTS-RT017A Adenovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
O le Human Adenovirus (HAdV) e a'afia i le ituaiga o Mammalian adenovirus, o se fa'ama'i DNA fa'alua fa'alua e aunoa ma se teutusi.Adenoviruses ua maua i le taimi nei e aofia ai le 7 subgroups (AG) ma le 67 ituaiga, lea e 55 serotypes e pathogenic i tagata.Faatasi ai ma i latou, e mafai ona oʻo atu i faʻamaʻi pipisi o le manava o le vaega B (ituaiga 3, 7, 11, 14, 16, 21, 50, 55), Vaega C (ituaiga 1, 2, 5, 6, 57) ma le Vaega E (ituaiga 4), ma e ono o'o atu ai ile manava manava manava ole Vaega F (ituaiga 40 ma le 41)[1-8].O ituaiga eseese e eseese uma auga o le falema'i, ae o le tele o fa'ama'i pipisi o le manava.O faʻamaʻi manava e mafua mai i faʻamaʻi pipisi o le tino o le tagata e tusa ma le 5% ~ 15% o faʻamaʻi manava o le lalolagi, ma le 5% -7% o faʻamaʻi manava o tamaiti i le lalolagi atoa [9].O le Adenovirus e afaina i le tele o vaega ma e mafai ona aʻafia i le tausaga atoa, aemaise lava i nofoaga tumutumu, lea e faigofie ona afaina i le lotoifale, aemaise lava i aʻoga ma tolauapiga a le militeri.
Auala
| FAM | adenovirus lautelenucleic acid |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Nasopharyngeal swab,Usu fa'a'i |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300Kopi/mL |
| Fa'apitoa | a) Faʻataʻitaʻi le faʻasalalauga faʻasalalau a le kamupani faʻamatalaga le lelei e le pusa, ma o le suʻega e fetaui ma manaʻoga. e) Fa'aoga le pusa lea e iloa ai ma e leai se fa'afeagai fa'atasi ma isi fa'ama'i fa'ama'i (e pei ole Influenza A virus, Influenza B virus, Respiratory syncytial virus, Parainfluenza virus, Rhinovirus, Human metapneumovirus, etc.) po'o siama (Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii, Staphylococcus aureus, ma isi). |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Monis (FQD-96A, HangzhouBioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Faiga PCR Taimi Moni, BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Galulue Galuega
(1) Fautuaina le fa'asusuina o mea e fa'aolaina:Macro & Micro-Su'ega Fa'ata'ita'i Fa'ata'ita'iga Reagent (HWTS-3005-8).E tatau ona faia le fa'aputuga e tusa ai ma fa'atonuga.O le fa'ata'ita'iga ua maua mai o ma'i'fa'ata'ita'iga o su'ega nasopharyngeal po'o le fa'a'i na aoina i luga ole nofoaga.Fa'aopoopo fa'ata'ita'iga i totonu o le fa'ata'ita'iga fa'asa'olotoga e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., vortex e fa'afefiloi lelei, tu'u i le vevela o le potu mo le 5 minute, ave i fafo ma fa'afefiloi lelei ina ia maua le DNA o fa'ata'ita'iga ta'itasi.
(2) Fautuaina le fa'asusuina o mea e fa'aolaina:Macro & Micro-Su'ega Viral Pusa DNA/RNA(HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).e tatau ona faia le taotoga e tusa ai ma faatonuga.O le voluma fa'ata'ita'i ua maua e 200μL, ma lele tele o le elution fautuainais80μL.
(3) Fautuaina le fa'asusuina o mea fa'asusu: Nucleic Acid Extraction po'o le Purification Reagent (YDP).315) saunia e Tiangen Biotech (Beijing) Co.,Ltd., o lee tatau ona faia le taotoga e tusa ai ma faatonuga.O le voluma fa'ata'ita'i ua maua e 200μL, ma lele tele o le elution fautuainais80μL.
