Mycobacterium Tuberculosis Nucleic Acid ma Rifampicin(RIF), Isoniazid Resistance(INH)

Fa'amatalaga Puupuu:

O lenei oloa e fetaui lelei mo le suʻesuʻeina lelei o le Mycobacterium tuberculosis DNA i faʻataʻitaʻiga sputum tagata i vitro, faʻapea foʻi ma le suiga o le homozygous i le 507-533 amino acid codon region (81bp, rifampicin resistance determining region) o le rpoB gene e mafua ai le Mycobacterium tuberculosis tetee rifampicin.

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Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid ma Rifampicin(RIF), Isoniazid Resistance (INH) Pusa Su'esu'ega (Melting Curve)

Epidemiology

O le Mycobacterium tuberculosis, e pei o le Tubercle bacillus (TB), o le siama faʻamaʻi e mafua ai le mamapala.I le taimi nei, o vailaʻau faʻasolosolo muamua e masani ona faʻaaogaina e aofia ai isoniazid, rifampicin ma ethambutol, ma isi. Ae ui i lea, ona o le le saʻo o le faʻaogaina o vailaʻau faʻamaʻi faʻamaʻi ma uiga o le fausaga puipui puipui o le mycobacterium tuberculosis, o le mycobacterium tuberculosis e atiaʻe ai le teteʻe o vailaʻau i vailaʻau faʻamaʻi, lea e aumaia ai luitau ogaoga i le puipuia ma le togafitia o le mamapala.

Auala

Igoa Sini			Tusitala	Quencher
Tali Fa'amauA	Tali Fa'amauB	Tali Fa'amauC	Tusitala	Quencher
rpoB 507-514	rpoB 513-520	IS6110	FAM	Leai
rpoB 520-527	rpoB 527-533	/	CY5	Leai
/	/	Pulea totonu	HEX(VIC)	Leai

Tali Fa'amauD	Tusitala	Quencher
InhA fa'alauiloa itulagi -15C>T, -8T>A, -8T>C	FAM	Leai
KatG 315 codon 315G>A、315G>C	CY5	Leai
itulagi fa'alauiloa AhpC -12C>T, -6G>A	ROX	Leai

Vaega Fa'atekinisi

Teuina	≤-18℃
Fa'asa-ola	12 masina
Ituaiga Fa'ata'ita'iga	fuga
CV	≤5.0%
LoD	Ole LoD ole mycobacterium tuberculosis ile atunuu ole 50 siama/mL.O le LoD o le rifampicin-resistant type wild type reference national 2×10³siama / mL, ma le LoD o le ituaiga mutant o le 2 × 10³siama/mL.Ole LoD ole siama ole isoniazid ile vao e 2x10³siama/mL, ma le LoD o siama mutant e 2x10³siama/mL.
Fa'apitoa	O fa'ai'uga o su'ega koluse na fa'ailoa mai ai e leai se tali fa'alava i le su'esu'eina o le genome o le tagata, isi fa'ama'i fa'ama'i e le tuberculosis mycobacteria ma le niumonia i lenei pusa;E le'i i ai se fa'afeagai fa'afeagai na maua i nofoaga o suiga o isi kenera e tete'e i vaila'au ile Mycobacterium tuberculosis.
Meafaigaluega Faatatau	SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Polokalama PCR Taimi Moni, Hangzhou Bioer technology QuantGene 9600 Real-Time PCR System, QuantStudio^®5 Fa'atonu PCR taimi moni.