Mycobacterium Tuberculosis Nucleic Acid ma Rifampicin(RIF), Resistance(INH)

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻaaogaina mo le suʻesuʻeina o le Mycobacterium tuberculosis DNA i totonu o le tagata, aganuʻu malosi (LJ Medium) ma le aganuʻu vai (MGIT Medium), sua o le bronchial lavage, ma suiga i le 507-533 amino acid codon region (81bp, rifampicin resistance determining region) o le faʻamaʻi faʻamaʻi faʻamaʻi faʻamaʻi o le Mycobacterium tuberculosis o le Mycobaccobaccin tuberculosis. i nofoaga autu mutation o Mycobacterium tuberculosis isoniazid resistance.E maua ai le fesoasoani i le suʻesuʻeina o le Mycobacterium tuberculosis faʻamaʻi, ma iloa ai le kenera tetee autu o le rifampicin ma isoniazid, lea e fesoasoani e malamalama ai i le fualaau faasaina o le Mycobacterium tuberculosis faʻamaʻi e le maʻi.

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HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid ma le Rifampicin(RIF), (INH) Pusa Su'esu'ega (Melting Curve)

Epidemiology

O le Mycobacterium tuberculosis, e pei o le Tubercle bacillus (TB), o le siama faʻamaʻi lea e mafua ai le mamapala, ma i le taimi nei, o vailaʻau e masani ona faʻaogaina muamua-laina anti-tuberculosis e aofia ai isoniazid, rifampicin ma ethambutol, ma isi.^[1]. Ae ui i lea, ona o le le saʻo o le faʻaogaina o vailaʻau faʻamaʻi faʻamaʻi ma uiga o le fausaga puipui o le mycobacterium tuberculosis lava ia, o le mycobacterium tuberculosis ua atiaʻe ai le teteʻe atu o vailaʻau i vailaʻau faʻamaʻi faʻamaʻi, ma o se faʻalavelave mataʻutia o le multidrug-resistant tuberculosis (MDR-TB), lea e teteʻe i vailaʻau e lua e sili ona taatele ma aoga, rifampicin ma o le rifampicin.^[2].

O le faʻafitauli o le faʻamaʻi pipisi o vailaʻau o loʻo i ai i atunuu uma na suʻesuʻeina e le WHO. Ina ia mafai ona tuʻuina atu fuafuaga faʻapitoa mo togafitiga mo tagata mamaʻi mamaʻi, e tatau ona iloa le teteʻe i vailaʻau faʻamaʻi faʻamaʻi, aemaise lava le tetee o le rifampicin, lea ua avea ma laasaga faʻamaonia e fautuaina e le WHO i le togafitiga o le mamapala.^[3]. E ui o le mauaina o le tete'e o le rifampicin e toetoe lava tutusa ma le mauaina o le MDR-TB, ae na'o le mauaina o le tete'e o le rifampicin e le amana'ia ai tagata mama'i e maua i le INH mono-tete'e (fa'asino i le tete'e i le isoniazid ae ma'ale'ale i le rifampicin) ma le mono-resistant rifampicin (ma'ale'ale i le isoniazid ae tete'e i le rifampicin), e ono o'o atu ai i tagata mama'i e faia muamua togafitiga le talafeagai. Ole mea lea, ole su'ega ole isoniazid ma le rifampicin ole su'ega ole la'ititi lea e mana'omia ile polokalame fa'atonutonu uma ole DR-TB^[⁴^].

Vaega Fa'ainisinia

Teuina	≤-18℃
Fa'asa-ola	12 masina
Ituaiga Fa'ata'ita'iga	Fa'ata'ita'i Sputum, Aganu'u Malosi (LJ Medium), Aganu'u Sua (MGIT Medium)
CV	<5.0%
LoD	Ole LoD ole pusa mo le su'eina ole Mycobacterium tuberculosis e 10 siama/mL;o le LoD o le pusa mo le sailia o le rifampicin ituaiga vao ma le mutant e 150 siama/mL; o le LoD o le pusa mo le suʻeina o le isoniazid ituaiga vao ma ituaiga mutant e 200 siama / mL.
Fa'apitoa	1) E leai se tali fa'afeagai pe a fa'aogaina le pusa e su'e ai DNA genomic tagata (500ng), isi 28 ituaiga o fa'ama'i fa'ama'i, ma 29 ituaiga o mycobacteria e le tuberculous (e pei ona fa'aalia i le Laulau 3).2) E leai se fa'afeagai fa'afeagai pe a fa'aogaina le pusa e su'e ai nofoaga o suiga o isi kenera e tete'e vaila'au o le rifampicin ma le isoniazid ma'ale'ale Mycobacterium tuberculosis (e pei ona fa'aalia i le Laulau 4).3) O mea masani faʻalavelave faʻalavelave i faʻataʻitaʻiga e suʻeina, e pei o le rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), derazinamide (45mg/L) fualaau faasaina, e leai se aafiaga i taunuuga o suega pusa.
Meafaigaluega Faatatau	SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Taimi Moni PCR System