Mycobacterium Tuberculosis INH Resistance

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻaaogaina e iloa lelei ai le suiga o le kenera 315th amino acid o le katG gene (K315G>C) ma le fesuiaiga o gene o le vaega faʻalauiloa o le InhA gene (- 15 C> T).


Fa'amatalaga Oloa

Faailoga o oloa

Igoa Oloa

HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)

Tusi Faamaonia

Myanmar FDA

Epidemiology

O le Isoniazid, o se vailaʻau autu e faʻamaʻi ai le mamapala na faʻalauiloaina i le 1952, o se tasi o vailaʻau sili ona aoga mo togafitiga tuʻufaʻatasi o le mamapala malosi ma se vailaʻau e tasi mo le mama mama.

O le KatG o le kenera autu e faʻapipiʻiina le catalase-peroxidase ma le katG gene mutation e mafai ona faʻalauteleina le faʻaogaina o le puipui o le mycolic acid, ma faʻamalosia ai le siama i le isoniazid.O le faʻamatalaga KatG e faʻamaopoopo le lelei ma suiga i le INH-MIC, ma o le faʻaititia o le 2-fold i le faʻamatalaga katG e maua ai le faʻateleina o le 2 faʻateleina i le MIC.O le isi mafua'aga ole tete'e o le isoniazid ile mycobacterium tuberculosis e tupu pe a fa'aofiina fa'avae, tape'ese po'o suiga e tupu ile InhA gene locus ole mycobacterium tuberculosis.

Auala

ROX inhA (-15C>T) site·
CY5

katG (315G>C) nofoaga

VIC (HEX)

IS6110

Vaega Fa'atekinisi

Teuina ≤-18℃ I le pogisa
Fa'asa-ola

12 masina

Ituaiga Fa'ata'ita'iga

Sputum

CV ≤5.0%
LoD

1 × 103siama/mL

Fa'apitoa E leai se koluse fa'aoso fa'atasi ma suiga o nofoaga e fa e tete'e ai vaila'au (511, 516, 526 ma le 531) o le kenera rpoB i fafo atu o le va'aiga o le pusa e iloa ai.

Meafaigaluega talafeagai:

Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni

Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi

QuantStudio®5 Faiga PCR Taimi Moni

SLAN-96P Faiga PCR Taimi Moni

LightCycler®480 Taimi moni PCR faiga

LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni

MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela

BioRad CFX96 Taimi Moni PCR System

BioRad CFX Opus 96 Taimi moni PCR System

Galulue Galuega

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