Mycobacterium Tuberculosis INH Resistance
Igoa Oloa
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)
Tusi Faamaonia
Myanmar FDA
Epidemiology
O le Isoniazid, o se vailaʻau autu e faʻamaʻi ai le mamapala na faʻalauiloaina i le 1952, o se tasi o vailaʻau sili ona aoga mo togafitiga tuʻufaʻatasi o le mamapala malosi ma se vailaʻau e tasi mo le mama mama.
O le KatG o le kenera autu e faʻapipiʻiina le catalase-peroxidase ma le katG gene mutation e mafai ona faʻalauteleina le faʻaogaina o le puipui o le mycolic acid, ma faʻamalosia ai le siama i le isoniazid.O le faʻamatalaga KatG e faʻamaopoopo le lelei ma suiga i le INH-MIC, ma o le faʻaititia o le 2-fold i le faʻamatalaga katG e maua ai le faʻateleina o le 2 faʻateleina i le MIC.O le isi mafua'aga ole tete'e o le isoniazid ile mycobacterium tuberculosis e tupu pe a fa'aofiina fa'avae, tape'ese po'o suiga e tupu ile InhA gene locus ole mycobacterium tuberculosis.
Auala
| ROX | inhA (-15C>T) site· |
| CY5 | katG (315G>C) nofoaga |
| VIC (HEX) | IS6110 |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ I le pogisa |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Sputum |
| CV | ≤5.0% |
| LoD | 1 × 103siama/mL |
| Fa'apitoa | E leai se koluse fa'aoso fa'atasi ma suiga o nofoaga e fa e tete'e ai vaila'au (511, 516, 526 ma le 531) o le kenera rpoB i fafo atu o le va'aiga o le pusa e iloa ai. Meafaigaluega talafeagai: Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
