Pusa Fa'amatalaga o le Dengue Virus I/II/III/IV Nucleic Acid (Fluorescence PCR)-RUO
Igoa o le oloa
Pusa Fa'amatalaga o le Dengue Virus I/II/III/IV Nucleic Acid (Fluorescence PCR)-RUO
Fa'ama'i pipisi
O le fiva dengue (DF), lea e mafua mai i le siama dengue (DENV), o se tasi lea o faʻamaʻi pipisi e sili ona taatele i le arbovirus. O lona auala e feaveaʻi ai e aofia ai le Aedes aegypti ma le Aedes albopictus. O le DF e taatele i vaega o le teropika ma le subtropical. O le DENV e aofia i le flavivirus i lalo o le flaviviridae, ma e mafai ona vaevaeina i ni serotypes e 4 e tusa ai ma le surface antigen. O faʻaaliga faʻapitoa o le siama DENV e aofia ai le tiga o le ulu, fiva, vaivai, faʻalauteleina o le lymph node, leukopenia ma isi mea, ma le toto, teʻi, manuʻa o le ate poʻo le oti foʻi i tulaga ogaoga. I tausaga talu ai nei, o suiga o le tau, le fausiaina o taulaga, le vave o le atinaʻeina o turisi ma isi mea ua saunia ai tulaga vave ma faigofie mo le feaveaʻiina ma le salalau o le DF, ma mafua ai ona faʻalauteleina pea le eria o le DF. O le mea lea, o le faʻatuina o se auala faigofie, patino ma vave e iloa ai le mafuaʻaga o le DENV e taua tele mo le suʻesuʻeina o le DF.
Fa'atulagaga Fa'apitoa
| Teuina | 2-8℃ |
| Aso e teu ai | 12 masina |
| Ituaiga Fa'ata'ita'iga | serum |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/mL |
| Fa'apitoa | E le lelei suega mo faʻataʻitaʻiga o le serum o le dengue virus ae leai se siama; o taunuuga o le suʻega faʻalavelave e faʻaalia ai afai e le sili atu le maualuga o le bilirubin i le serum i le 168.2μmol/mL, o le maualuga o le hemoglobin e gaosia e le hemolysis e le sili atu i le 130g/L, o le maualuga o le lipid i le toto e le sili atu i le 65mmol/mL, ma o le aofaʻi atoa o le IgG i le serum e le sili atu i le 5mg/mL, e leai se aafiaga i le iloa o le siama dengue; faʻaaoga le siama Hepatitis A, siama Hepatitis B, siama Hepatitis C, siama Herpes, siama Eastern equine encephalitis, siama Hantaan, siama Bunia, siama West Nile, siama Zika ma faʻataʻitaʻiga o le serum genomic a le tagata mo le suʻega cross-reactivity, ma o taunuuga e faʻaalia ai e leai se cross reaction i le va o lenei pusa ma siama o loʻo i luga. |
| Meafaigaluega Fa'aoga | Ituaiga I: Fa'aoga Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Ituaiga II: EudemonTMAIO800 (HWTS-EQ007) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Tafega o Galuega
E manaʻomia e vailaʻau faʻamaʻi ae leʻi tuʻuina atu:
Ituaiga I: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (e mafai ona fa'aaogaina fa'atasi ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
Ituaiga II: Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), e mafai ona faʻaaogaina faʻatasi ma le EudemonTM AIO800 (HWTS-EQ007) mai le Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Aʻo leʻi faʻaaogaina le vailaʻau suʻega Ituaiga II, e iai i le tagata faʻatau se vailaʻau lagolago e faʻaoso ai ma se meafaigaluega faʻateleina, ma o le a taʻitaʻia e le inisinia le tagata faʻatau e faʻatino le faʻatinoga faʻataʻitaʻi pe a faʻaaogaina mo le taimi muamua.
Mea fa'aalu e mana'omia ae le'i tu'uina atu:DNase/RNase Free Tips, totigilima e lafoa'i, paipa DNase/RNase Free EP, centrifuge, tulaga fa'amagnetiki, fasi paipa e 8 mo le PCR.
