4 ituaiga o siama o le manava
Igoa o le oloa
HWTS-RT099- 4 ituaiga o siama manava Nucleic Acid Detection Kit (Fluorescence PCR)-内参是NED-ABI 7500 Real-Time PCR System/ABI 7500 Faiga PCR Vave i le Taimi Moni/QuantStudio®5 Faiga PCR Taimi Moni
HWTS-RT158-4 Ituaiga o Vaila'au Fa'ama'i Manava Nucleic Acid Detection Kit (Fluorescence PCR)-内参Quasar 705
Fa'ama'i pipisi
O le Fa'ama'i o le Corona Virus 2019, e ta'ua o le "COVID-19", e faasino i le niumonia e mafua mai i2009-nCoVfa'ama'i pipisi.2009-nCoVo se siama o le coronavirus e a'afia i le ituaiga β. O le COVID-19 o se fa'ama'i pipisi fa'afuase'i i le manava, ma e masani ona a'afia tagata. I le taimi nei, o le puna o le fa'ama'i o gasegase ua a'afia i le2009-nCoV, ma o tagata e leai ni faʻailoga o loʻo aʻafia e ono avea foʻi ma puna o le faʻamaʻi. E faʻatatau i suʻesuʻega faʻamaʻi o loʻo iai nei, o le vaitaimi e faʻatupu ai le faʻamaʻi e 1-14 aso, e tele lava 3-7 aso. O le fiva, tale mago ma le vaivai o faʻailoga autū ia. E toʻaitiiti ni gasegase sa iai ni faʻailoga.e pei oisu pupuni, isu tafe, tiga o le fa'a'ī, tiga o le tino ma le manava tata, ma isi mea faapena.
Alavai
| FAM | 2019-nKoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Pulega i Totonu |
Fa'atulagaga Fa'apitoa
| Teuina | -18℃ |
| Aso e teu ai | 9 masina |
| Ituaiga Fa'ata'ita'iga | Su'isu'i o le gutu (oropharyngeal swab) |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Kopi/mLVairasi o le Influenza A/Vairasi o le Influenza B/Vairasi o le Respiratory syncytial: 500 Kopi/mL |
| Fa'apitoa | a) O fa'ai'uga o le cross-reactivity e fa'aalia ai e leai se cross reaction i le va o le kit ma le human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, human pulmonary virus, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ma newborn cryptococcus ma le human genomic nucleic acid. b) Malosiaga e tete'e atu ai i fa'alavelave: filifili le mucin (60mg/mL), 10% (v/v) o le toto ma le phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (e aofia ai ma mea fa'asao) (20 mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ma le tobramycin (0.6mg/mL) mo le su'ega fa'alavelave, ma o taunu'uga e fa'aalia ai o mea fa'alavelave e iai le tele o vaila'au o lo'o ta'ua i luga e leai se tali fa'alavelave i taunu'uga o su'ega o siama. |
| Meafaigaluega Fa'aoga | Fa'aogaina o le Biosystems 7500 Real-Time PCR SystemFa'aogaina o Biosystems 7500 Faiga PCR Vave i le Taimi Moni Faiga PCR Taimi Moni a le QuantStudio®5 |
Tafega o Galuega
Filifiliga 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) na gaosia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. O le aofa'i o le fa'ata'ita'iga na fa'a'esea e 200μL, ma le aofa'i fautuaina o le elution e 80μL.
Filifiliga 2.
O le QIAamp Viral RNA Mini Kit (52904) na gaosia e le QIAGEN po'o le Nucleic Acid Extraction or Purification Kit (YDP315-R) na gaosia e le Tiangen Biotech (Beijing) Co., Ltd. O le aofa'i o le fa'ata'ita'iga na fa'a'esea e 140μL, ma le aofa'i fautuaina o le elution e 60μL.
