4 Ituaiga Vairusi Manava
Igoa o oloa
HWTS-RT099- 4 Ituaiga Fa'ama'i Vairusi Nucleic Acid Detection Kit (Fluorescence PCR)-内参是NED-ABI 7500 Real-Time PCR System/ABI 7500 Fa'atonuga PCR Taimi Moni Anapogi/QuantStudio®5 Faiga PCR Taimi Moni
HWTS-RT158-4 Ituaiga Fa'ama'i Vairusi Pusa Su'esu'ega Nucleic Acid (Fluorescence PCR)-内参Quasar 705
Epidemiology
Corona Virus Disease 2019, e ta'ua o le "COVID-19", e faasino i le niumonia e mafua mai i2009-nCoVfa'ama'i.2009-nCoVo se coronavirus e a'afia i le ituaiga β. O le COVID-19 ose fa'ama'i fa'ama'i pipisi, ma e masani ona a'afia le faitau aofa'i. I le taimi nei, o le mafuaʻaga o faʻamaʻi pipisi e masani lava o tagata mamaʻi ua aʻafia e2009-nCoV, ma tagata a'afia e leai ni fa'aaliga e mafai fo'i ona avea ma puna o fa'ama'i. Faʻavae i luga o suʻesuʻega faʻamaʻi o loʻo iai nei, o le taimi faʻamaʻi e 1-14 aso, tele 3-7 aso. O le fiva, tale mago ma le vaivai o fa'aaliga autu ia. O nai tagata gasegase sa i ai ni fa'ailogae pei opipii isu, tafe isu, tiga fa'a'i, myalgia ma manava manava, ma isi.
Auala
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | -18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Kopi/mLFa'ama'i o le Influenza A/Varusi Fa'ama'i B/Varusi fa'atasi ma le manava: 500Kopi/mL |
| Fa'apitoa | a) O fa'ai'uga fa'asagatau fela'ua'i e fa'aalia ai e leai se tali fa'afeagai i le va o le pusa ma le coronavirus a le tagata SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, Emochlamyne virus enterovirus, humanydia virus enterovirus A, B, C, virus umochlamyne. B, C, D, virus pulmonary tagata, epstein-barr virus, misle virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus, klebberobakte pneumoniae, streptococcus, klebberobakte pneumoniae asu aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ma cryptococcus faatoa fanau mai ma tagata genomic acid nucleic. b) Faʻalavelave faʻalavelave faʻalavelave: filifili mucin (60mg / mL), 10% (v / v) o le toto ma le phenylephrine (2mg / mL), oxymetazoline (2mg / mL), sodium chloride (e aofia ai mea faʻasao) (20 mg / mL), beclomethasone (20mg / mL), dexanisg/mL (20mg/mL), dexanisolimethasone (20mg/mL) triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/mL) (1mg/mL), lopinavir(500mg/mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ma le. O fa'ai'uga e fa'aalia ai o mea fa'alavelave fa'atasi ma fa'atonuga o lo'o ta'ua i luga e leai se tali fa'alavelave i fa'ai'uga o su'ega a fa'ama'i. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR SystemFa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni |
Galulue Galuega
Filifiliga 1.
Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) gaosia e Jiangsu Macro & Micro-Test Med-Tech0μL samples o le volumes fautuaina o le Med-Tech0, Ltd. voluma e 80μL.
Filifiliga 2.
QIAamp Viral RNA Mini Kit (52904) gaosia e le QIAGEN poʻo le Nucleic Acid Extraction poʻo le Purification Kit (YDP315-R) na gaosia e Tiangen Biotech (Beijing) Co., Ltd. O le faʻataʻitaʻiga o le voluma o le 140μL, ma o le voluma elution fautuaina o le 60μL.
