29 Ituaiga Fa'ama'i Fa'ama'i Fa'atasiga Nucleic Acid
Igoa o oloa
HWTS-RT160 -29 Ituaiga Fa'ama'i Fa'ama'i Tu'ufa'atasia Nucleic Acid Detection Kit
Epidemiology
O fa'ama'i fa'ama'i o le fa'ama'i taatele lea i tagata, e mafai ona tupu i so'o se itupa, tausaga ma le itulagi. O se tasi lea o mafuaʻaga autu o le faʻamaʻi ma le oti i le faitau aofaʻi i le lalolagi atoa[1]. O fa'ama'i pipisi masani e aofia ai le novel coronavirus, Influenza A virus, Influenza B virus, Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, ma Streptococcus pneumoniae, ma isi[2,3]. O faʻailoga ma faʻailoga e mafua mai i faʻamaʻi pipisi e foliga tutusa, ae o auala togafitiga, aoga ma le ala o faʻamaʻi pipisi e mafua mai i siama eseese e eseese [4,5]. I le taimi nei, o auala autu o loʻo faʻaaogaina i totonu o fale suʻesuʻe e suʻesuʻe ai faʻamaʻi pipisi o loʻo taʻua i luga e aofia ai: virus isolation, antigen detection and nucleic acid detection, ma isi. O fa'ai'uga leaga e le aofia ai fa'ama'i pipisi o le manava ma e le tatau ona fa'aaogaina e na'o le fa'avae mo su'esu'ega, togafitiga, po'o isi fa'ai'uga tau pulega. O se i'uga lelei e le mafai ona fa'asalaina ai fa'ama'i siama po'o fa'ama'i fefiloi e isi virusi o lo'o i fafo atu o fa'ailoga o su'ega. E tatau ona maua e le au fai fa'ata'ita'i a'oa'oga fa'apolofesa i le fa'atuputeleina o kene po'o le su'esu'eina o le biology mole, ma iai ni fa'ailoga talafeagai fa'atino fa'ata'ita'iga. E tatau i le falesu'esu'e ona iai ni nofoaga talafeagai mo le puipuiga o le ola ma le puipuiga.
Vaega Fa'atekinisi
| Teuina | -18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Usu fa'a'i |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Kopi/μL |
| Fa'apitoa | O fa'ai'uga o su'esu'ega fa'afeagai na fa'aalia ai e leai se fa'afeagai i le va o lenei pusa ma le Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilarclatrium strains of Moraxalis. mamapala, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholdery cepatum, Burkholdery cepatum. marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii, ma tagata genomic nucleic acid. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni, Fa'aaogāina Biosystems 7500 Va'ai Va'aiga Fa'atonu PCR, QuantStudio®5 Faiga PCR Taimi Moni, SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.), LightCycler®480 taimi moni PCR faiga, LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekonolosi), MA-6000 Moni-Taimi Fa'atauva'a Fa'a'oloa'ele'ele (Suzhou Molarray Co., Ltd.), BioRad CFX96 Polokalama PCR Taimi Moni, BioRad CFX Opus 96 Taimi moni PCR System. |
Galulue Galuega
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ma le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (lea e mafai ona fa'aogaina e Euch)TM AIO800 (HWTS-EQ007)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
O le voluma faʻataʻitaʻiga na maua mai o le 200μL ma le faʻataʻitaʻiga faʻapitoa e 150μL.
