Herpes Simplex Virus Type 1/2，(HSV1/2) Nucleic Acid

Fa'amatalaga Puupuu:

O lenei pusa o lo'o fa'aogaina mo le su'esu'eina o le Herpes Simplex Virus Type 1 (HSV1) ma le Herpes Simplex Virus Type 2 (HSV2) e fesoasoani i le su'esu'eina ma le togafitia o tagata mama'i e masalomia fa'ama'i HSV.

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Fa'amatalaga Oloa

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Igoa o oloa

HWTS-UR018A-Herpes simplex virus type 1/2, (HSV1/2) pusa su'esu'e o le nucleic acid (Fluorescence PCR)

Epidemiology

O faʻamaʻi pipisi mai feusuaiga (STD) o loʻo avea pea ma se tasi o faʻamataʻu tele i le saogalemu o le soifua maloloina lautele o le lalolagi.O ia fa'ama'i e mafai ona o'o atu ai i le le maua o fanau, fa'ato'a fanau mai o le fetal, tumo ma fa'alavelave matuia eseese.E tele ituaiga o STD pathogens, e aofia ai siama, viruses, chlamydia, mycoplasma ma spirochetes, e aofia ai Neisseria gonorrhoeae, Mycoplasma genitalium, Chlamydia trachomatis, HSV1, HSV2, Mycoplasma hominis, ma Ureaplasma urealyticum e taatele.

O le fa'ama'i fa'afeusuaiga ose fa'ama'i pipisi fa'afeusuaiga e mafua mai ile HSV2, lea e sili ona fa'ama'i.I tausaga talu ai nei, o le aʻafiaga o herpes genital ua matua faʻateleina, ma ona o le faʻateleina o amioga faʻafeusuaiga mataʻutia, o le fua faatatau o le suʻesuʻeina o le HSV1 i le herpes genital ua faʻateleina ma lipotia mai e maualuga i le 20% -30%.O se fa'ama'i muamua i le fa'ama'i o le genital herpes e tele lava ina le leoa e aunoa ma ni fa'ailoga fa'apitoa fa'apitoa se'i vagana herpes i le lotoifale i le mucosa po'o le pa'u o nai gasegase.Talu ai ona o le fa'ama'i fa'afeusuaiga o lo'o fa'aalia e ala i le fa'amaligiina o viral i le olaga atoa ma le fa'asolosolo i le toe tupu, e taua le siakiina o siama i se taimi vave e mafai ai ma poloka lona feavea'i.

Auala

FAM	HSV1
CY5	HSV2
VIC(HEX)	Pulea i totonu

Vaega Fa'atekinisi

Teuina	Suavai: ≤-18℃ I le pogisa
Fa'asa-ola	12 masina
Ituaiga Fa'ata'ita'iga	urethral secretions, fa'alilo fa'atagata
Ct	≤38
CV	≤5.0%
LoD	50 Kopi/tali
Fa'apitoa	E leai se feso'ota'iga fa'atasi ma isi fa'ama'i STD e pei o Treponema pallidum, Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Mycoplasma genitalium, ma Ureaplasma urealyticum.
Meafaigaluega Faatatau	E mafai ona fetaui ma meafaifa'aili PCR fa'aulu i luga o le maketi. Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio^®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler^®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System

Galulue Galuega

Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Fa'ata'ita'i Fa'ata'ita'iga Reagent (HWTS-3005-8).E tatau ona matua fa'atino le fa'aputuga e tusa ai ma fa'atonuga.

Fautuaina le fa'asusuina o mea fa'apipi'i: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona fa'aoga ile Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona matua faʻatinoina le faʻaaogaina e tusa ai ma faatonuga.Ole maualuga ole elution fautuaina e tatau ona 80 μL.

Fautuaina le fa'a'ese'esega: Nucleic Acid Extraction po'o le Purification Reagent(YDP315) e Tiangen Biotech(Beijing) Co.,Ltd.E tatau ona matua fa'atino le fa'aputuga e tusa ai ma fa'atonuga.Ole maualuga ole elution fautuaina e tatau ona 80 μL.

Muamua: Fa'atasiga Fa'ama'i Manava

Sosoo ai: Mycoplasma Hominis Nucleic Acid

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