Virus Ebola Zaire
Igoa o oloa
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
O le Ebola virus e a'afia i le Filoviridae, o se fa'ama'i RNA e le'i tu'ufa'atasia e tasi le fa'ai'uga leaga.Virus o filaments uumi ma le averesi virion umi o le 1000nm ma le lautele e tusa ma le 100nm.O le Ebola virus genome o se RNA e le'i tu'ufa'atasia fa'atasi ma le tele o le 18.9kb, fa'ailoga 7 porotini fa'avae ma le 1 polotini e le fa'atulagaina.Ebola virus e mafai ona vaevaeina i ituaiga e pei o Zaire, Sudan, Bundibugyo, Tai Forest ma Reston.Faatasi ai ma i latou, o le ituaiga Zaire ma le ituaiga Sudan ua lipotia mai e mafua ai le tele o tagata maliliu mai siama.O le EHF (Ebola Hemorrhagic Fever) o se fa'ama'i fa'ama'i pipisi e mafua mai i le virusi o le Ebola.E masani ona a'afia tagata i le fa'afeso'ota'i ma sua o le tino, mea lilo ma fe'umaga a tagata mama'i po'o manu fa'ama'i, ma o fa'aaliga ile falema'i e tele lava ina fa'aoso ile fiva, tafetoto ma le tele o totoga fa'aleagaina.O le EHF o loʻo i ai le maualuga o le maliu o le 50% -90%.
Auala
| FAM | MP nucleic acid |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Sua fou, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/μL |
| Fa'apitoa | Fa'aoga pusa e su'e ai fa'amatalaga leaga a le kamupani, o taunu'uga e fetaui ma mana'oga. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Polokalama PCR Taimi Moni, ma BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Galulue Galuega
Filifiliga 1.
Fautuaina le fa'aaogaina o le fa'aputuga: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R) e Tiangen Biotech (Beijing) Co., Ltd.E tatau ona maua mai e tusa ai ma le faʻatonuga, ma o le faʻataʻitaʻiga fautuaina o le faʻataʻitaʻiga o le 140μL ma o le voluma elution fautuaina o le 60μL.
Filifiliga 2.
Fautuaina le fa'aaogaina o mea fa'apitoa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).It e tatau ona aveese mai e tusa ai ma faatonuga.O le faʻataʻitaʻiga faʻataʻitaʻiga voluma o 200μL, ma le voluma elution fautuaina e 80μL.
Filifiliga 3.
Fautuaina le fa'aaogaina o mea fa'apitoa: Nucleic Acid Extraction Reagent (1000020261) ma le High Throughput Automated Sample Sample System(MGISP-960) e le BGI e tatau ona fa'ailoa mai e tusa ai ma fa'atonuga.Ole voluma fa'asili ole 160μL, ma o le voluma elution fautuaina ole 60μL.
