Staphylococcus Aureus ma le Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Igoa o oloa
HWTS-OT062 Staphylococcus Aureus ma le Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
Staphylococcus aureus o se tasi o siama faʻamaʻi taua o faʻamaʻi pipisi o le nosocomial.Staphylococcus aureus (SA) e a'afia i le staphylococcus ma o se sui o siama Gram-positive, lea e mafai ona maua ai le tele o vaila'au oona ma enzymes osofa'i.Ole siama ei ai uiga ole tufatufa lautele, malosi faʻamaʻi ma le maualuga o le tetee.Thermotable nuclease gene (nuc) o se kenera e sili ona fa'asaoina ole staphylococcus aureus.
Auala
| FAM | gene mecA e tetee i le methicillin |
| ROX | Pulea i totonu |
| CY5 | staphylococcus aureus nuc gene |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ & puipuia mai le malamalama |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | sputum, pa'u ma aano vaivai fa'ata'ita'iga fa'ama'i fa'ama'i, ma fa'ata'ita'iga o su'ega isu |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL siama e tetee i le methicillin.A iloa e le pusa le LoD fa'asinomaga a le atunu'u, 1000/mL staphylococcus aureus e mafai ona iloa. |
| Fa'apitoa | O le su'ega fa'afeagai e fa'aalia ai o lenei pusa e leai se fa'afeagai fa'afeagai ma isi fa'ama'i manava e pei o le methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiumaniella, pneumonia. mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e mafai ona fa'aogaina ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).Fa'aopoopo le 200µL o le masima masani i le fa'agasologa fa'agaioiina, ma o la'asaga mulimuli ane e tatau ona toso mai e tusa ai ma fa'atonuga, ma o le voluma elution fautuaina o le 80μL.
Filifiliga 2.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Faaopopo le 1mL o le masima masani i le paʻu pe a uma ona fufulu ile saline masani, ona faʻafefiloi lelei.Centrifuge i le 13,000r/min mo le 5 minute, aveese le supernatant (fa'aagaga 10-20μL o le supernatant), ma mulimuli i fa'atonuga mo le su'eina mulimuli ane.
Fautuaina le fa'aputuina o mea fa'aputu: Nucleic Acid Extraction po'o le Purification Reagent (YDP302) e Tiangen Biotech (Beijing) Co.,Ltd.E tatau ona fa'atinoina le fa'atosina e tusa ai ma le Laasaga 2 o le tusi lesona.E fautuaina le fa'aogaina o le RNase ma le DNase-leai se vai mo le fa'afefe ma le voluma o le 100μL.
