Ono Fa'ama'i Manava
Igoa o le oloa
HWTS-RT175-Ono Pusa e Iloa ai le Nucleic Acid o Fa'ama'i Manava (Fluorescence PCR)
Fa'ama'i pipisi
O faʻamaʻi pipisi o le manava o le vaega sili ona taatele o faʻamaʻi o tagata e mafai ona tutupu i soʻo se itupa, matua ma le eria faʻafanua ma o se tasi lea o mafuaʻaga sili ona taua o le maʻi ma le oti i tagata i le lalolagi atoa. O siama masani o le manava e aofia ai le respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) ma le Mycoplasma pneumoniae. O faʻailoga ma aʻafiaga faʻapitoa e mafua mai i faʻamaʻi pipisi o le manava e tutusa lava, ae o togafitiga, aoga, ma le umi o le faʻamaʻi e eseese i faʻamaʻi e mafua mai i siama eseese. I le taimi nei, o auala autu mo le suʻesuʻeina o siama o le manava o loʻo taʻua i luga e aofia ai: le vavaeʻeseina o siama, le suʻesuʻeina o antigen ma le suʻesuʻeina o nucleic acid. O lenei pusa e fesoasoani i le suʻesuʻeina o faʻamaʻi pipisi o le manava e ala i le suʻesuʻeina ma le faʻailoaina o siama nucleic acid faʻapitoa i tagata taʻitoʻatasi e iai faʻailoga ma aʻafiaga o faʻamaʻi pipisi o le manava, faʻatasi ai ma isi suʻesuʻega faʻapitoa ma suʻesuʻega i le fale suʻesuʻe.
Fa'atulagaga Fa'apitoa
| Teuina | ≤-18℃ |
| Aso e teu ai | 12 masina |
| Ituaiga Fa'ata'ita'iga | Fa'ata'ita'iga o le swab o le gutu (oropharyngeal swab) |
| Ct | Adv, PIV, MP, RhV, hMPV, RSV Ct≤38 |
| CV | <5.0% |
| LoD | O le LoD o Adv, MP, RSV, hMPV, RhV ma le PIV e 200 Kopi/mL uma. |
| Fa'apitoa | O taunuuga o le suega o le cross-reactivity na faaalia ai e leai se cross-reactivity i le va o le kit ma le novel coronavirus, influenza A virus, influenza B virus, human bocavirus, cytomegalovirus, herpes simplex virus type 1, varicella zoster virus, EBV, pertussis bacillus, Chlamydophila pneumoniae, Corynebacterium spp, Escherichia coli, Haemophilus influenzae, Lactobacillus spp, Legionella pneumophila, C. catarrhalis, ma isi siama ua faavaivaia o Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria spp, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus salivarius, Actinobacillus baumannii, Narrow-feeding maltophilic monococci, Burkholderia maltophilia, Streptococcus striatus, Nocardia sp., Sarcophaga viscosa, Citrobacter citriodora, Cryptococcus spp, Aspergillus fumigatus, Aspergillus flavus, Pneumatobacteria spp, Candida albicans, Rohypnogonia viscera, streptococci oral, Klebsiella pneumoniae, Chlamydia psittaci, Rickettsia q fever ma human genomic nucleic acids. Malosiaga e tete'e atu ai i fa'alavelave: mucin (60 mg/mL), toto o le tagata, benfotiamine (2 mg/mL), oxymetazoline (2 mg/mL), sodium chloride (20 mg/mL), beclomethasone (20 mg/mL), dexamethasone (20 mg/mL), flunitrazolone (20 μg/mL), triamcinolone acetonide (2 mg/mL), budesonide (1 mg/ mL), mometasone (2 mg/mL), fluticasone (2 mg/mL), histamine hydrochloride (5 mg/mL), tui puipuia o le siama fulū ola i totonu o le isu, benzocaine (10%), menthol (10%), zanamivir (20 mg/mL), ribavirin (10 mg/L), paramivir (1 mg/mL), oseltamivir (0.15 mg/mL), mupirocin (20 mg/mL), tobramycin (0.6 mg/mL), Na faia se suega fa'alavelave i le UTM, saline, guanidine hydrochloride (5 M/L), Tris (2 M/L), ENTA-2Na (0.6 M/L), trilostane (15%), isopropyl alcohol (20%), ma le potassium chloride (1 M/L), o taunuuga na faaalia ai e leai se tali fa'alavelave i taunuuga o le iloa o le pathogen i le maualuga o mea fa'alavelave o lo'o i luga. |
| Meafaigaluega Fa'aoga | SLAN-96P Polokalame PCR Taimi Moni Fa'aogaina o le Biosystems 7500 Real-Time PCR System QuantStudio®5 Faiga PCR Taimi Moni Polokalama Iloa PCR Taimi Moni a le LineGene 9600 Plus MA-6000 Fa'ata'amilosaga Fa'avevela Fa'atatau i le Taimi Moni BioRad CFX96 Polokalame PCR Taimi Moni BioRad CFX Opus 96 Polokalame PCR Taimi Moni |
Tafega o Galuega
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e mafai ona fa'aaogaina fa'atasi ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B))e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e fautuaina mo le aveeseina o faʻataʻitaʻiga ma lee tatau ona faia laasaga o loʻo mulimuli maita'ita'igafa'atulagaina e tusa ai ma le IFUo le Kit.
