Fa'atasiga Fa'ama'i Manava
Igoa o oloa
HWTS-RT158A Pusa Su'esu'ega Tu'ufa'atasi Fa'ama'i manava (Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
Corona Virus Disease 2019, e ta'ua o'KOVITI 19', e faasino i le niumonia e mafua mai ile 2019-nCoV.O le 2019-nCoV o se coronavirus e a'afia i le ituaiga β.O le COVID-19 ose fa'ama'i fa'ama'i pipisi, ma e masani ona a'afia le faitau aofa'i.I le taimi nei, o le mafuaʻaga o faʻamaʻi pipisi e masani lava o tagata mamaʻi ua aʻafia i le 2019-nCoV, ma o tagata aʻafia e leai ni faʻamaʻi e mafai foi ona avea ma puna o faʻamaʻi.Faʻavae i luga o suʻesuʻega faʻamaʻi o loʻo iai nei, o le taimi faʻamaʻi e 1-14 aso, tele 3-7 aso.O le fiva, tale mago ma le vaivai o fa'aaliga autu ia.O nai tagata mama'i na iai fa'ailoga e pei o le pipii o le isu, tafe le isu, tiga fa'a'i, myalgia ma le manava, ma isi.
O le fulū, e masani ona ta'ua o le "flu", ose fa'ama'i fa'ama'i fa'ama'i pipisi e mafua mai i le fulū.E maualuga tele le pipisi.E masani lava ona feavea'i e ala i le tale ma le mafatua.E masani ona oso i le tautotogo ma le taumalulu.O fa'ama'i fa'ama'i e vaevaeina i le fulū A (IFV A), fa'ama'i B (IFV B), ma le fa'ama'i C (IFV C) e tolu ituaiga, e a'afia uma i siama pipii, e mafua ai fa'ama'i o le tagata ma'i mo siama A ma le B, e tasi lava. -pa'ia, RNA virusi vaega.Ole fa'ama'i ole Influenza A ose fa'ama'i pipisi tele ile manava, e aofia ai le H1N1, H3N2 ma isi fa'ama'i laiti, ia e faigofie ona suia ma fa'ama'i i le lalolagi atoa.O le "Shift" e faasino i le suiga o le influenza A virus, e mafua ai ona aliaʻe se virus fou "subtype".O fa'ama'i fa'ama'i fa'ama'i B ua vaevaeina i gafa e lua, Yamagata ma Vitoria.O le fa'ama'i o le Influenza B e na'o le antigenic drift, ma e 'alo'ese mai le mata'ituina ma le fa'ate'aina a le tagata e ala i ona suiga.Ae ui i lea, o le saoasaoa o le evolusione o le influenza B virus e telegese nai lo le virus influenza A tagata.Ole fa'ama'i ole Influenza B e mafai fo'i ona fa'atupu fa'ama'i ile manava ole tagata ma ta'ita'i atu ai ile fa'ama'i.
Respiratory syncytial virus (RSV) o se siama RNA, e patino i le aiga paramyxoviridae.E feavea'i e ala i matāua o le ea ma feso'ota'i vavalalata ma o le fa'ama'i autu lea o fa'ama'i pipisi o le manava maualalo i pepe.O pepe e a'afia ile RSV e ono atia'e le bronchiolitis tuga ma le niumonia, lea e feso'ota'i ma le sela i tamaiti.O pepe e iai auga ogaoga, e aofia ai le fiva maualuga, rhinitis, pharyngitis ma laryngitis, ona sosoo ai lea ma le bronchiolitis ma le niumonia.O nai tamaiti mama'i e mafai ona faigata ile otitis media, pleurisy ma myocarditis, ma isi.
Auala
| FAM | SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| Kuasar 705 | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | -18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300Kopi/mL Fa'ama'i Fa'ama'i A/Varusi Fa'ama'i B/Varusi fa'atasi ma le manava: 500Kopi/mL |
| Fa'apitoa | a) Fa'ai'uga fa'asagatau fela'ua'i e fa'aalia ai e leai se fa'afeagai i le va o le pusa ma le coronavirus tagata SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus tagata, enterovirus A, B, C, D, epstein-barr virus, misle virus, cytomegalo virus tagata, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, asu aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ma tagata faatoa fanau mai genococcus nut. e) Fa'alavelave fa'alavelave: filifili mucin (60mg/mL), 10% (v/v) o le toto ma le phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (e aofia ai mea fa'asao) (20mg/mL) , beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL) , histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL). ), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) ma le tobramycin (0.6mg/mL) ) mo su'ega fa'alavelave, ma o fa'ai'uga o lo'o fa'aalia ai o mea fa'alavelave fa'atasi ma fa'atonuga o lo'o ta'ua i luga e leai se tali fa'alavelave i fa'ai'uga o su'ega a fa'ama'i. |
| Meafaigaluega Faatatau | BioRad CFX96 Taimi Moni PCR System Rotor-Gene Q 5plex HRM Platform taimi moni PCR System |
