Clostridium difficile toxin A/B gene（C.diff）
Igoa o oloa
HWTS-OT031A Nucleic Acid Detection Kit mo Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
Clostridium difficile (CD), o le kalama-positive anaerobic sporogenic Clostridium difficile, o se tasi o faʻamaʻi autu e mafua ai faʻamaʻi pipisi o le intestinal nosocomial.I le falemaʻi, e tusa ma le 15% ~ 25% o le manava e fesoʻotaʻi ma le antimicrobial, 50% ~ 75% o le antimicrobial-related colitis ma le 95% ~ 100% o le pseudomembranous enteritis e mafua mai i le Clostridium difficile infection (CDI).Clostridium difficile o se faʻamaʻi faʻapitoa, e aofia ai faʻamaʻi oona ma faʻamaʻi e le o ni toxigenic.
Auala
| FAM | tcdAgene |
| ROX | tcdBgene |
| VIC/HEX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | nofoa |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Fa'apitoa | fa'aoga lenei pusa e su'e ai isi fa'ama'i o le intestinal e pei ole Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus and human genomic DNA, o taunuuga e leaga uma. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni(FQD-96A,HangzhouBioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Faʻaopopo le 180μL o le lysozyme buffer i le faʻafefe (faʻafefe le lysozyme i le 20mg / mL ma le lysozyme diluent), paipa e faʻafefiloi lelei, ma faʻagasolo i le 37 ° C mo le sili atu i le 30 minute. Ave le 1.5mL o le RNase / DNase-free centrifuge tube, ma faaopoopo180μL o le pulea lelei ma le leaga le pulea i le faasologa.Faaopoopo10μL o le pulea i totonu i le faʻataʻitaʻiga e faʻataʻitaʻiina, pulea lelei, ma le lelei le pulea i le faasologa, ma faʻaaoga le Nucleic Acid Extraction poʻo le Purification Reagent (YDP302) e Tiangen Biotech (Beijing) Co., Ltd. mo le faʻataʻitaʻiga mulimuli ane o DNA, ma fa'amolemole mulimuli lelei i fa'atonuga mo le fa'aoga mo la'asaga fa'apitoa.Fa'aaoga le DNase/RNase free H2O mo elution, ma o le voluma elution fautuaina o le 100μL.
Filifiliga 2.
Ave le 1.5mL o le RNase/DNase-free centrifuge tube, ma faaopoopo 200μL o le pulea lelei ma le lelei le pulea i le faasologa.Faaopoopo10μL o le pulea i totonu i le faʻataʻitaʻiga e faʻataʻitaʻiina, pulea lelei, ma le le lelei o le pulea i le faasologa, ma faʻaoga le Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).E tatau ona faʻatinoina le faʻaaogaina e tusa ai ma le faʻatonuga mo le faʻaaogaina, ma o le voluma elution fautuaina o le 80μL.
