Clostridium difficile toxin A/B gene(C.diff)

Fa'amatalaga Puupuu:

O lenei pusa ua fa'amoemoe mo le su'esu'eina fa'apitoa in vitro o le clostridium difficile toxin A gene ma le toxin B i fa'ata'ita'iga o le fe'ai mai tagata mama'i ua masalomia fa'ama'i clostridium difficile.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-OT031A Nucleic Acid Detection Kit mo Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)

Tusi Faamaonia

CE

Epidemiology

Clostridium difficile (CD), o le kalama-positive anaerobic sporogenic Clostridium difficile, o se tasi o faʻamaʻi autu e mafua ai faʻamaʻi pipisi o le intestinal nosocomial.I le falemaʻi, e tusa ma le 15% ~ 25% o le manava e fesoʻotaʻi ma le antimicrobial, 50% ~ 75% o le antimicrobial-related colitis ma le 95% ~ 100% o le pseudomembranous enteritis e mafua mai i le Clostridium difficile infection (CDI).Clostridium difficile o se faʻamaʻi faʻapitoa, e aofia ai faʻamaʻi oona ma faʻamaʻi e le o ni toxigenic.

Auala

FAM tcdAgene
ROX tcdBgene
VIC/HEX Pulea i totonu

Vaega Fa'atekinisi

Teuina

≤-18℃

Fa'asa-ola 12 masina
Ituaiga Fa'ata'ita'iga nofoa
Tt ≤38
CV ≤5.0%
LoD 200CFU/mL
Fa'apitoa fa'aoga lenei pusa e su'e ai isi fa'ama'i o le intestinal e pei ole Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus and human genomic DNA, o taunuuga e leaga uma.
Meafaigaluega Faatatau Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni

Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi

QuantStudio®5 Faiga PCR Taimi Moni

SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.)

LightCycler®480 Taimi moni PCR faiga

LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni(FQD-96A,HangzhouBioer tekinolosi)

MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.)

BioRad CFX96 Taimi Moni PCR System

BioRad CFX Opus 96 Taimi moni PCR System

Galulue Galuega

Filifiliga 1.

Faʻaopopo le 180μL o le lysozyme buffer i le faʻafefe (faʻafefe le lysozyme i le 20mg / mL ma le lysozyme diluent), paipa e faʻafefiloi lelei, ma faʻagasolo i le 37 ° C mo le sili atu i le 30 minute. Ave le 1.5mL o le RNase / DNase-free centrifuge tube, ma faaopoopo180μL o le pulea lelei ma le leaga le pulea i le faasologa.Faaopoopo10μL o le pulea i totonu i le faʻataʻitaʻiga e faʻataʻitaʻiina, pulea lelei, ma le lelei le pulea i le faasologa, ma faʻaaoga le Nucleic Acid Extraction poʻo le Purification Reagent (YDP302) e Tiangen Biotech (Beijing) Co., Ltd. mo le faʻataʻitaʻiga mulimuli ane o DNA, ma fa'amolemole mulimuli lelei i fa'atonuga mo le fa'aoga mo la'asaga fa'apitoa.Fa'aaoga le DNase/RNase free H2O mo elution, ma o le voluma elution fautuaina o le 100μL.

Filifiliga 2.

Ave le 1.5mL o le RNase/DNase-free centrifuge tube, ma faaopoopo 200μL o le pulea lelei ma le lelei le pulea i le faasologa.Faaopoopo10μL o le pulea i totonu i le faʻataʻitaʻiga e faʻataʻitaʻiina, pulea lelei, ma le le lelei o le pulea i le faasologa, ma faʻaoga le Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).E tatau ona faʻatinoina le faʻaaogaina e tusa ai ma le faʻatonuga mo le faʻaaogaina, ma o le voluma elution fautuaina o le 80μL.

 


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