Mycoplasma Pneumoniae （MP）
Igoa o oloa
HWTS-RT024 Mycoplasma Pneumoniae（MP） Nucleic Acid Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) o se ituaiga o microorganism prokaryotic laʻititi, o loʻo i le va o siama ma siama, e iai le fausaga o le sela ae leai se puipui. O le MP e masani ona mafua ai le faʻamaʻi o le manava o tagata, aemaise lava i tamaiti ma tupulaga talavou. E mafai ona mafua ai le niumonia mycoplasma tagata, fa'ama'i o le manava o tamaiti ma le niumonia atypical. O faʻaaliga faʻapitoa e eseese, o le tele o ia mea e ogaoga tale, fiva, maʻalili, ulu tiga, tiga faʻaʻi. O fa'ama'i pipisi i luga o le manava ma le bronchial pneumonia e sili ona taatele. O nisi gasegase e mafai ona atia'e mai fa'ama'i pipisi i luga o le manava i luga i le niumonia tuga, fa'alavelave ogaoga fa'alenava ma e ono tupu ai le oti.
Auala
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Sputum, Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Kopi/mL |
| Fa'apitoa | a） Gaoioiga koluse: e leai se fegalegaleaiga faafeagai ma Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneinosaophila. Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus and human genomic nucleic acid. b） Malosiaga e tetee atu ai: e leai se faʻalavelave pe a suʻeina mea faʻalavelave faʻatasi ma faʻatonuga nei: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) toto o le tagata, levofloxacin (10μg/mL), moxifloxacin(0. (80μg/mL), azithromycin (1mg/mL), clarithromycin(125μg/mL), erythromycin(0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
(1) Fa'ata'ita'iga Sputum
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (0H6C Acid Extractor) HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Fa'aopoopo le 200µL o le masima masani i le su'ega fa'apipi'i. E tatau ona faia le su'ega mulimuli ane e tusa ai ma faatonuga mo le fa'aoga. O le voluma elution fautuaina o le 80µL. Fautuaina le fa'asusuina o le su'ega: Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R). E tatau ona faia sa'o le fa'aaogaina e tusa ai ma fa'atonuga mo le fa'aoga. Ole voluma fa'amama fa'atonu e 60µL.
(2) Usu ole fa'aa'ai
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (0H6C Acid Extractor) HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faia le suʻega e tusa ai ma faatonuga mo le faʻaaogaina. Ole fua ole fa'ata'ita'iga fautuaina ole 200µL, ma le voluma elution fautuaina ole 80μL. Reagent fa'asao fautuaina: QIAamp Viral RNA Mini Kit (52904) po'o le Nucleic Acid Extraction or Purification Reagent (YDP315-R). E tatau ona faia sa'o le fa'aaogaina e tusa ai ma fa'atonuga mo le fa'aoga. Ole volume ole fa'ata'ita'iga fautuaina ole 140µL, ma le voluma fa'ata'ita'i fautuaina ole 60μL.
