Mycobacterium Tuberculosis Nucleic Acid ma Rifampicin Resistance

Fa'amatalaga Puupuu:

O lenei pusa e fetaui lelei mo le suʻesuʻeina o le Mycobacterium tuberculosis DNA i faʻataʻitaʻiga sputum tagata i vitro, faʻapea foʻi ma le suiga o le homozygous i le 507-533 amino acid codon region o le rpoB gene lea e mafua ai le Mycobacterium tuberculosis rifampicin resistance.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid ma le Rifampicin Resistance Detection Pusa (Melting Curve)

Tusi Faamaonia

CE

Epidemiology

Mycobacterium tuberculosis , e le'i umi ae pei o le Tubercle bacillus, TB, o le siama fa'ama'i e mafua ai le mamapala. I le taimi nei, o vailaʻau faʻamaʻi pipisi e masani ona faʻaaogaina muamua e aofia ai isoniazid, rifampicin ma hexambutol, ma isi. O le mycobacterium tuberculosis e atia'e ai le tete'e atu i vaila'au e tetee atu i le mamapala, lea e aumaia ai lu'itau ogaoga i le puipuia ma le togafitia o le mamapala.

O le Rifampicin na fa'aaogaina lautele i togafitiga o gasegase mamapala talu mai le tuai o 1970s, ma e iai sona aafiaga taua. O le filifiliga muamua lava e fa'apu'upu'u le chemotherapy o tagata mama'i mamapala. O le tetee i le Rifampicin e mafua ona o le suiga o le kenera rpoB. E ui lava o loʻo faʻaauau pea ona faʻaalia fualaau fou e tetee atu ai i le mamapala, ma o loʻo faʻaauau pea le faʻaleleia atili o le aoga o tagata mamaʻi mamaʻi, o loʻo i ai pea le le lava o vailaʻau faʻamaʻi, ma o le tulaga o le faʻaogaina o fualaau faʻatauvaʻa e fai si maualuga. E manino lava, o le Mycobacterium tuberculosis i tagata mamaʻi e maua i le mama mama e le mafai ona faʻaumatia atoa i se taimi talafeagai, lea e iu ai ina oʻo atu i tikeri eseese o vailaʻau faʻasaina i le tino o le tagata maʻi, faʻaumiina le ala o le faʻamaʻi, ma faʻateleina ai le lamatiaga o le oti o le tagata maʻi.

Auala

Auala

Auala ma Fluorophores

Reaction Buffer A

Fa'asagaga Pa'u B

Fa'asagaga Pa'u C

FAM Channel

Tusitala: FAM, Quencher: Leai

rpoB 507-514

rpoB 513-520

38KD ma le IS6110

CY5 Alavai

Tusitala: CY5, Quencher: Leai

rpoB 520-527

rpoB 527-533

/

HEX (VIC) Auala

Tusitala: HEX (VIC), Quencher: Leai

Pulea totonu

Pulea totonu

Pulea totonu

Vaega Fa'ainisinia

Teuina

≤-18℃ I le pogisa

Fa'asa-ola

12 masina

Ituaiga Fa'ata'ita'iga

Sputum

CV

≤5.0%

LoD

mycobacterium tuberculosis 50 siama/mL

ituaiga vao e tetee i le rifampicin: 2x103siama/mL

mutant homozygous: 2x103siama/mL

Fa'apitoa

E iloa ai le mycobacterium tuberculosis vao ma nofoaga o suiga o isi genes tete'e vaila'au e pei o le katG 315G>C\A, InhA-15C> T, o fa'ai'uga o su'ega e leai se tete'e i le rifampicin, o lona uiga e leai se fa'afeagai fa'asagatau.

Mea faigaluega talafeagai:

SLAN-96P Faiga PCR Taimi Moni

BioRad CFX96 Taimi Moni PCR System

LightCycler480® Polokalama PCR Taimi Moni

Galulue Galuega

Afai e faʻaaoga le Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (lea e mafai ona faʻaogaina i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-300TS)-3 Micro-Test Viral DNA/RNA Column(HWTS-3022-50) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. mo le aveesea, faaopoopo 200μL o le Pulea Lelei, pulea lelei ma faagasologa sputum sample e tofotofoina i le faasologa, ma faaopoopo 10μL o le pulea totonu eseese, le lelei ma le faagasologa Positive i totonu o le faʻataʻitaʻiga Positive. o laasaga mulimuli e tatau ona matua faʻatinoina e tusa ai ma faʻatonuga o le aveeseina. O le voluma faʻataʻitaʻiga na maua mai o le 200μL, ma o le faʻataʻitaʻiga faʻapitoa e 100μL.


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