Klebsiella Pneumoniae, Acinetobacter Baumannii ma Pseudomonas Aeruginosa ma Vailaau Fa'asagaga Fa'asaina (KPC, NDM, OXA48 ma IMP) Multiplex
Igoa o oloa
HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii ma Pseudomonas Aeruginosa ma Vailaau Fa'asagaga Fa'asaina (KPC, NDM, OXA48 ma IMP) Pusa Su'esu'ega Tele (Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
Klebsiella pneumoniae ose fa'ama'i fa'ama'i masani ma o se tasi o siama fa'ama'i taua e mafua ai fa'ama'i pipisi. A faʻaitiitia le malosi o le tino, e ulu atu le siama i totonu o mama mai le manava, ma mafua ai le faʻamaʻi i le tele o vaega o le tino, ma o le vave faʻaaogaina o vailaau faʻamaʻi o le ki lea e faʻamalolo ai [1]. E masani ona faʻatasi ma isi faʻamaʻi pipisi ma faʻamaʻi pipisi, faʻatasi ai ma uiga o le maualuga o le faʻamaʻi ma le maualuga o le maliu. Pseudomonas aeruginosa o le sili ona taatele e le faʻafefeteina gram-negative bacilli i le falemaʻi faʻataʻitaʻiga, ma o se pathogen opportunistic taua mo faʻamaʻi pipisi i le falemaʻi, faʻatasi ai ma uiga o le faigofie colonization, fesuiaiga faigofie ma multi-drug tetee.
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Sputum |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 kopi/mL |
| Fa'apitoa | a) O le suʻega faʻafefeteina o loʻo faʻaalia ai o lenei pusa e leai se faʻalavelave faʻafefe ma isi faʻamaʻi manava, e pei ole Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella Escherichudomona fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus ma sputum samples e aunoa ma ni sini, ma isi. b) Faʻalavelave faʻalavelave: Filifili mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, ma roxithromycin, ma isi mo le suʻega faʻalavelave, ma o faʻaiʻuga o loʻo faʻaalia ai o mea faʻalavelave faʻalavelave o loʻo taʻua i luga e le mafai ona maua le niumonia. Acinetobacter baumannii, Pseudomonas aeruginosa ma carbapenem teteʻe genes KPC, NDM, OXA48 ma IMP. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni, Fa'aaogāina Biosystems 7500 Va'ai Va'aiga Fa'atonu PCR, QuantStudio®5 Faiga PCR Taimi Moni, LightCycler®480 taimi moni PCR faiga, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, Bioer technology), MA-6000 Moni-Taimi Fa'atauva'a Fa'a'oloa'ele'ele (Suzhou Molarray Co., Ltd.), BioRad CFX96 Polokalama PCR Taimi Moni, BioRad CFX Opus 96 Taimi moni PCR System. |
Galulue Galuega
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (lea e mafai ona faʻaaogaina ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. e fautuaina mo le faʻataʻitaʻiga o le faʻataʻitaʻiga o laʻasaga e tatau ona faʻataʻitaʻiina i le faʻataʻitaʻiga o le IFU o laʻasaga e tatau ona faia e tusa ai ma le faʻataʻitaʻiga o laʻasaga o le IFU ma le laʻasaga pito i lalo.
