Fa'ama'i Fulu A/ Fa'ama'i Fulu B
Igoa o oloa
HWTS-RT174-Influenza A Virus/ Influenza B Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Faʻavae i luga o le eseesega o le antigenic i le va o le NP gene ma le M gene, e mafai ona vaevaeina faʻamaʻi pipisi i ituaiga e fa: faʻamaʻi A (IFV A), faʻamaʻi B (IFV B), faʻamaʻi C (IFV C) ma le siama D (IFV D).[1]. O le fa'ama'i o le Influenza A e tele 'au ma serotypes lavelave, ma e mafai ona maua le malosi e sosolo atu i 'au e ala i le toe tu'ufa'atasiga o kenera ma suiga fa'afetaui. E le maua e tagata le puipuiga tumau i le influenza A virus, o lea e masani ona a'afia tagata o vaitausaga uma. Ole fa'ama'i ole Influenza A ole fa'ama'i autu e mafua ai fa'ama'i fulū[2]. Ole fa'ama'i ole Influenza B e tele lava ina fa'ateleina i se vaega la'ititi ma o le taimi nei e leai ni vaega laiti. O mea autu e mafua ai le faʻamaʻi o tagata o le gafa B/Yamagata poʻo le gafa B/Victoria. Faatasi ai ma tulaga faʻamaonia o le fulū i atunuu e 15 i le itulagi o Asia ma le Pasefika i masina taʻitasi, o le fua faʻamaonia o le influenza B virus e 0-92%[3]. E le pei o le influenza A virus, o vaega patino e pei o tamaiti ma tagata matutua e faigofie ona maua i le influenza B virus ma e faigofie i faʻalavelave faʻalavelave, lea e faʻatupuina ai se avega sili atu i sosaiete nai lo le siama A.[4].
Auala
| FAM | MP nucleic acid |
| ROX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab sample |
| Ct | Fulu A, Fulu BCt≤35 |
| CV | <5.0% |
| LoD | Fulu A ma le Fulu Be 200Kopi/mL uma |
| Fa'apitoa | Fa'asagaga fa'afeagai: E leai se fa'afeagai i le va o le pusa ma le Bocavirus, rhinovirus, cytomegalovirus, respiratory syncytial virus, parainfluenza virus, Epstein-Barr virus, herpes simplex virus, Varicella-zoster virus, mumps virus, enterovirus, misles virus, human metapneumovirus, adenovirus, coronavirus human, MERSrovirus, coronavirus virus, MERSrovirus tagata, coronavirus rotavirus, MERSrovirus. pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis carinii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, Neisseria gonorrhoeanas, Candida glaurous, Candida glaucoma, Candida glaucoma, Candida glaucoma, Candida glaucoma, Candida glauces Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium, ma DNA genomic tagata. Su'ega fa'alavelave: Filifili le mucin (60 mg/mL), toto o le tagata (50%), phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (20mg/mL) fa'atasi ai ma le 5% fa'asao, beclomethasone (20mg/mL), dexamethasone (20mg/mL), trinisolide (20mg/mL), flunisolide (20mg/mL) (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpiromL (20mg/mL), tomybramL (20mg/mL), mucinpirocin (20mg/mL). oseltamivir (60ng/mL), ribavirin (10mg/L) mo suʻesuʻega faʻalavelave, ma faʻaiʻuga e faʻaalia ai o mea faʻalavelave i faʻamaʻi o loʻo i luga e le faʻalavelave i le mauaina o le pusa. |
| Meafaigaluega Faatatau | SLAN-96P Faiga PCR Taimi Moni Applied Biosystems 7500 Real-Time PCR System QuantStudio®5 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.o lo'o fautuaina mo le fa'ata'ita'iga fa'ata'ita'iga ma lee tatau ona i ai laasaga mulimuli aneta'ita'ie tusa ai ma le IFUo le Pusa.
