Dengue Virus, Zika Virus ma Chikungunya Virus Multiplex
Igoa o oloa
HWTS-FE040 Dengue Virus, Zika Virus ma Chikungunya Virus Multiplex Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
O le fiva Dengue (DF), lea e a'afia e le dengue virus (DENV), o se tasi lea o fa'ama'i pipisi fa'ama'i pipisi.O lona ala fa'asalalau e aofia ai Aedes aegypti ma Aedes albopictus.O le DF e masani lava ona taatele i nofoaga vevela ma vaega laiti.DENV e a'afia i le flavivirus i lalo o le flaviviridae, ma e mafai ona fa'avasegaina i 4 serotypes e tusa ai ma le antigen i luga.O fa'aaliga fa'alema'i ole fa'ama'i ole DENV e tele lava ina aofia ai le ulu, fiva, vaivai, fa'alatele ole pona o le lymph, leukopenia ma isi mea, ma le toto, te'i, manu'a ate po'o le oti i tulaga ogaoga.I tausaga talu ai nei, o suiga o le tau, taulaga, atinaʻe vave o tagata tafafao maimoa ma isi mea na maua ai le sili atu le vave ma le talafeagai tulaga mo le faʻasalalauina ma le faʻasalalauina o le DF, e oʻo atu ai i le faʻalauteleina o le faʻamaʻi o le DF.
Auala
| FAM | MP nucleic acid |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | -18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Sua fou |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/mL |
| Fa'apitoa | O faʻaiʻuga o suʻega faʻalavelave e faʻaalia ai pe a le sili atu i le 168.2μmol / ml le maualuga o le bilirubin i totonu o le toto, o le hemoglobin faʻatupuina e le hemolysis e le sili atu i le 130g / L, o le toto lipid concentration e le sili atu i le 65mmol / ml, o le aofaʻi IgG. O le fa'atonuga o le toto e le sili atu nai lo le 5mg/mL, e leai se a'afiaga i le siama dengue, fa'ama'i Zika po'o le su'esu'eina o le virus chikungunya.Fa'ama'i Ate A, fa'ama'i Hepatitis B, fa'ama'i Hepatitis C, fa'ama'i Herpes, fa'ama'i o le Encephalitis equine i Sasa'e, fa'ama'i o Hantavirus, fa'ama'i o Bunya, fa'ama'i o le West Nile ma fa'ata'ita'iga o le toto o le tagata ua filifilia mo le su'ega cross-reactivity, ma o fa'ai'uga e fa'aalia ai e leai se felafolafoaiga i le va o lenei pusa ma pathogens o loʻo taʻua i luga. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
TIANamp Virus DNA/RNA Kit (YDP315-R), ma e tatau ona fa'atinoina le fa'auigaina e tusa ai ma fa'atonuga mo le fa'aoga.O le voluma faʻataʻitaʻiga na maua mai o le 140μL, ma o le voluma elution fautuaina o le 60μL.
Filifiliga 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona faʻaaogaina ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ma e tatau ona faia le faʻaaogaina e tusa ai ma faatonuga mo le faʻaaogaina.O le voluma faʻataʻitaʻiga na maua mai o le 200μL, ma le faʻataʻitaʻiga o le elution voluma e 80μL.
