Hepatitis B Virus RNA
Igoa o oloa
HWTS-HP007 Virus Hepatitis B RNA Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
QOle su'esu'ega ole HBV-RNA ile serum e mafai ona sili atu ona mata'ituina le maualuga ole HBV cccDNA ile hepatocytes, ma i le taimi lava e tasi e aloese mai le fa'aleagaina tele o gasegase.E taua tele mo le suʻesuʻeina fesoasoani o le siama o le hepatitis B (HBV) faʻamaʻi ma le mataʻituina lelei ma le faʻamavaeina o vailaʻau faʻamaʻi o le NAs antiviral therapy i gasegase CHB.
Auala
| FAM | HBV-RNA |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | sua |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 100 kopi/mL |
| Fa'apitoa | O faʻaiʻuga o suʻega faʻalavelave e faʻaalia ai e leai se aʻafiaga i le fuafuaina o le HBV RNA pe a le sili atu i le 168.2μmol / mL le maualuga o le bilirubin i le toto, o le faʻaogaina o le hemoglobin e gaosia e le hemolysis e le sili atu i le 130g/L, ma le o le faʻaogaina o gaʻo toto e le sili atu i le 65mmol/mL;e leai se a'afiaga i le fa'amautuina o le HBV RNA pe a le sili atu i le 5mg/mL le aofa'i ole IgG ile serum.O fa'ai'uga o su'ega fa'afeagai e fa'afeagai ai e fa'aalia ai e leai ni fa'alavelave fa'asaga i le va o lenei pusa ma isi fa'ama'i po'o siama e maua e le nucleic acid i fa'ata'ita'iga toto (virus hepatitis C, cytomegalovirus tagata, virus epstein-barr, virus immunodeficiency tagata, virus hepatitis A, syphilis. , virus herpes tagata ituaiga 6, herpes simplex virus type 1, herpes simplex virus type 2, influenza A virus, propionibacterium acnes, staphylococcus aureus, candida albicans) ma le genomes o le tagata;pe a uma ona ave e tagata mamaʻi le lamivudine, telbivudine, adefovir dipivoxil ma le entecavir, pe a oʻo i luga o le uta viral o le hepatitis B i le tino le maualalo o le LoD o le pusa, e mafai lava ona iloa lelei le pusa. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR SystemFa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) |
Galulue Galuega
Filifiliga 1.
Viral DNA/RNA Isolation Kit (NA007-2) e le GenMag Biotechnology Co., Ltd. O laasaga fa'apitoa e fa'ate'aina fa'amolemole mulimuli i le tusi lesona.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona faʻaaogaina ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. O le faʻaaogaina e tatau ona faia e tusa ai ma le faʻatonuga tusi lesona, o le faʻataʻitaʻiga faʻataʻitaʻiga voluma o le 200μL, ma le voluma elution fautuaina o le 80μL.
