Fa'ama'i Hepatitis B DNA Fa'afuainumera Fluorescence
Igoa o oloa
HWTS-HP015 Virus Hepatitis B DNA Fua'i Fua'i Fu'a Su'esu'ega Pusa (Fluorescence PCR)
Epidemiology
O le Hepatitis B o se fa'ama'i e mafua mai i le virusi o le hepatitis B (HBV), e masani ona fa'aalia i manu'a o le ate, ma e mafai ona fa'aleagaina le tele o totoga.O tagata mama'i Hepatitis B o lo'o fa'aalia i le falema'i e pei o le vaivai, leai se fia'ai, pito i lalo po'o le edema lautele, ma le hepatomegaly ona o le fa'aletonu o le ate.E lima pasene o tagata matutua ua a'afia ma le 95% o tagata ua a'afia i luga o le sa'o e le mafai ona fa'amama lelei le HBV, e i'u ai i fa'ama'i fa'ama'i faifai pea, ma o nisi fa'ama'i fa'ama'i e i'u ina tupu a'e i le ate cirrhosis ma le hepatocellular carcinoma.[1-4].
Auala
| FAM | HBV-DNA |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | sua fou, Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Fa'apitoa | O fa'ai'uga fa'apitoa o lo'o fa'aalia ai o mataupu uma e 50 o fa'ata'ita'iga serum leaga HBV DNA soifua maloloina e leaga;o fa'ai'uga o su'ega fa'afeagai e fa'aalia ai e leai se fa'afeagai i le va o lenei pusa ma isi fa'ama'i (HAV, HCV, DFV, HIV) mo le su'esu'eina o le nucleic acid ma fa'ata'ita'iga toto, ma genomes a tagata. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E tatau ona faʻatinoina le faʻaaogaina e tusa ai ma le tusi faʻatonuga, o le faʻataʻitaʻiga faʻataʻitaʻiga o le 300μL, ma o le voluma elution fautuaina o le 70μL.
