Afete A Virus
Igoa o oloa
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Fa'ama'i Hepatitis A (HAV) o le mafua'aga autu lea o fa'ama'i pipisi fa'ama'i.O le virusi o se fa'ama'i lelei-sense tasi-stranded RNA virus ma e patino i le Hepadnavirus genus o le aiga Picornaviridae.O le siama o le Hepatitis A, e masani lava ona feaveaʻi e ala ile fecal-oral route, e tetee ile vevela, acids, ma le tele o solvents organic, e mafai ona ola mo se taimi umi i figota, vai, eleele, poʻo palapala alititai[1-3].E feavea'i e ala i mea'ai po'o vai ua pisia, pe tu'u sa'o mai le tagata i le tagata.O mea'ai e feso'ota'i ma le HAV e aofia ai faisua ma pipi, strawberries, raspberries, blueberries, aso, fuala'au lanu meamata, ma tamato fa'amago [4‒6].
Auala
| FAM | HAV nucleic acid |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | Suavai: 9 masina, Lyophilized: 12 masina |
| Ituaiga Fa'ata'ita'iga | Serum/stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 2 Kopi/μL |
| Fa'apitoa | Fa'aaoga pusa e su'e ai isi fa'ama'i hepatitis e pei ole hepatitis B, C, D, E, enterovirus 71, virus coxsackie, virus Epstein-Barr, norovirus, HIV ma le genome o le tagata. E leai se fa'alavelave fa'asagatau. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekonolosi), MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Fa'ata'ita'iga o le sua
Filifiliga 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E tatau ona aveese mai e tusa ai ma faatonuga.Ole voluma fa'amama fa'atonu e 80µL.
Filifiliga 2.
TIANamp Virus DNA/RNA Kit (YDP315-R) gaosia e Tiangen Biotech (Beijing) Co., Ltd. E tatau ona aveese mai e tusa ai ma faatonuga.O le voluma fa'ata'ita'iga na maua mai o le 140μL.Ole voluma fa'amama fa'atonu e 60µL.
2.Fa'ata'ita'i Fa'ata'oto
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E tatau ona aveese mai e tusa ai ma faatonuga.Ole voluma fa'amama fa'atonu e 80µL.
