Chlamydia Trachomatis fa'amago fa'a'aisa
Igoa o oloa
HWTS-UR032C/D-Chlamydia Trachomatis fa'amago fa'a'aisa Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) o se ituaiga o microorganism prokaryotic e matua faʻamaʻi i sela eukaryotic.[1].Chlamydia trachomatis ua vaevaeina i AK serotypes e tusa ai ma le auala serotype.O faʻamaʻi pipisi o le urogenital e tele lava ina mafua mai i le trachoma biological variant DK serotypes, ma o tane e tele lava ina faʻaalia e pei o le urethritis, lea e mafai ona faʻamalolo e aunoa ma se togafitiga, ae o le tele o ia mea e faʻamaʻi, faʻalavelave faʻafuaseʻi, ma e mafai ona tuʻufaʻatasia ma epididymitis, proctitis, ma isi.[2].O fafine e mafai ona mafua mai i le urethritis, cervicitis, ma isi, ma sili atu faʻalavelave faʻalavelave o salpingitis[3].
Auala
| FAM | Chlamydia trachomatis (CT) |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤30 ℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Su'e fa'atama'ita'i Usu urethral tane Mimi tane |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 Kopi/mL |
| Fa'apitoa | e leai se fa'afeagai fa'afeagai i le va o lenei pusa ma isi fa'ama'i fa'ama'i fa'ama'i e pei ole papillomavirus ituaiga tagata 16, ituaiga papillomavirus tagata 18, ituaiga virus Herpes simplex Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium epidermidiscoccus. , Escherichia coli, Gardnerella vaginalis, Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei ma DNA genomic tagata, ma isi. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Polokalama PCR Taimi Moni ma BioRad CFX Opus 96 Faiga PCR Taimi Moni Faigofie Amp Fa'aola-taimi Fluorescence Isothermal Detection System(HWTS-1600）. |
Galulue Galuega
Filifiliga 1.
Macro & Micro-Su'ega Fa'ata'ita'i Fa'ata'ita'iga Reagent (HWTS-3005-8).E tatau ona fa'ataunu'uina le fa'aputuga e tusa ma le IFU.Fa'aopoopo le DNA fa'ata'ita'i na maua mai i le fa'ata'ita'iga fa'asa'olotoga i totonu o le tali fa'apolopolo ma fa'ata'ita'i sa'o le meafaigāluega, po'o fa'ata'ita'iga ua maua e tatau ona teu i le 2-8 ℃ mo le sili atu i le 24 itula.
Filifiliga 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E tatau ona faʻatinoina le faʻaaogaina e tusa ai ma le IFU, ma o le volume elution fautuaina o le 80μL.Ole DNA fa'ata'ita'i na maua mai ile faiga fa'amaneta e fa'avevela ile 95°C mo le 3 minute ona ta'ele loa lea ole aisa mo le 2 minute.Fa'aopoopo le DNA fa'ata'ita'i ua mae'a fa'agaoioi i totonu o le tali fa'apolopolo ma fa'ata'ita'i le meafaigāluega po'o fa'ata'ita'iga fa'ata'ita'i e tatau ona teuina i lalo -18°C mo le le sili atu i le 4 masina.Ole numera ole fa'a'aisa fa'alua ma le liusua e le tatau ona sili atu ile 4 ta'amilosaga.
