Faaliliuga-mago le chlamydia tchoomamas
Igoa o oloa
Hwts-ur032C / d-Faʻatasi-mago chlamydia trachmatis nuckic acid dictict boad (enzymatic probe isothermation)
Leitio
Chlamydia tchochomas (ct) o se ituaiga o procharyotic microorganism e matua faigata lava i le okarayotic sela[1]. ChimyDia Trachmatis vaevaeina i Ak shatrotypes tusa ma le srotype metotia. O le orrogeenitisi o faʻamaʻi o loʻo mafua mai e mafua mai i le trachoma bigy dk srontles davers darty[2]. O tamaʻitaʻi e mafai ona mafua ai i urethritis, cervitic, ma isi mea, ma sili atu ogaoga faʻafitauli o le masima[3].
Auala
| Oge | Chlamydia tchochomas (ct) |
| Rox | Pusa i totonu |
Tulaga faapitoa
| Teuina | ≤30 ℃ |
| Fata-ola | 12 masina |
| Primetien ituaiga | Tamaitai Cervical Swab Tane urethral swab Tane mimiti |
| Tt | ≤28 |
| CV | ≤10.0% |
| Lote | 400 kopi / ml |
| Faʻapitoa | E leai se satauro-o le faʻaaogaina i le va o lenei pusa ma isi itupa o loʻo i ai i le sili atu o le maualuga o le papillomavirus i le ituaiga, o le a le Heroplasma. , Esterichia Coli, Gardnelagi Vaigasis, Smican Abimastis, lactobacincys Cortovis, Ascebacuss damic dna, ma isi |
| Talosaga talafeagai | Faʻaaogaina Biossisystem 7500 le moni-taimi PCR System Faʻaaogaina Biossisys 7500 Suʻega Moni-Taimi PCR Aofai®5 moni-taimi pcr faiga Slan-96p moni-taimi pcr polokalama (Hentshi Medical Technical Co., Ltd.) Malamalama lelei®480 moni-taimi pcr system Linegene 9600 Ma le taimi moni-taimi pcr dictiction system (fqd-96a, chanzhou bioer tekonolosi) Ma-6000 moni-taimi le aofaiga o le karmlel cymlel Biorad cfx96 moni-taimi pcr system ma biorad cfx optus 96 moni-taimi pcr faiga Faigofie amp moni-taimi fua o le le maua(Hwts-1600). |
Galuega tafe
Filifiliga 1.
Macro & Micro-Suʻega faʻataʻitaʻiga o loʻo faʻamatuʻu atu le toe faʻaaogaina (HWTS-3005-8). O le soʻotaga e tatau ona faia i le agavaʻa e tusa ai ma le antou. Faʻaopopo le faʻataʻitaʻiga DNA e aveʻesea e le faʻataʻitaʻiga faʻatagaina i totonu o le tali atu buffer ma suʻega i luga o le upega tafailagi tuʻusaʻo, poʻo le faʻaopopoina o faʻataʻitaʻiga e tatau ona teuina i le 2-8 ℃ mo le 24 itula.
Filifiliga 2.
Macro & Micro-Suʻega General PRO / RNA ORN (HWTS-3017-50, hwts-3017-32, Hwts-3017-48, Hwts-3017-96) ma macro & Micro-Suʻega otometi Nuckic Acad Expractor (HWTS-3006C, Hwts-300600). O le soʻona faʻamalosia e tatau ona faʻatinoina i le tulaga e tusa ai ma le afai, ma le fautuaina o le tali mai o le 80 viome. O le faʻataʻitaʻiga DNA na faʻaopopoina e le maneta Bead Metotia o loʻo vevela i le 95 ° C mo 3 minute ma le vave aisa-puao ai mo le 2 minute. Faʻaopopo le faʻagaioiga o le DNA i le tali a le BUFFER ma le suʻega i luga o le mea faigaluega poʻo le faʻagaioiga o loʻo faʻagaioia e tatau ona teuina i lalo -18 ° C mo le 4 masina. O le numera o le toe faia o le faʻaʻaisa ma le 'ai e le tatau ona sili atu ma le 4 cycles.
