Chlamydia Trachomatis fa'amago fa'a'aisa
Igoa o oloa
HWTS-UR032C/D-Chlamydia Trachomatis fa'amago fa'a'aisa Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)
Epidemiology
Chlamydia trachomatis (CT) o se ituaiga o microorganism prokaryotic e matua faʻamaʻi i sela eukaryotic.[1]. Chlamydia trachomatis ua vaevaeina i AK serotypes e tusa ai ma le auala serotype. O faʻamaʻi pipisi o le urogenital e tele lava ina mafua mai i le trachoma biological variant DK serotypes, ma o tane e tele lava ina faʻaalia e pei o le urethritis, lea e mafai ona faʻamalolo e aunoa ma se togafitiga, ae o le tele o ia mea e faʻamaʻi, faʻalavelave faʻafuaseʻi, ma e mafai ona tuʻufaʻatasia ma epididymitis, proctitis, ma isi.[2]. O fafine e mafai ona mafua mai i le urethritis, cervicitis, ma isi, ma sili atu faʻalavelave faʻalavelave o salpingitis[3].
Auala
| FAM | Chlamydia trachomatis (CT) |
| ROX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤30 ℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Su'e fa'atama'ita'i Usu urethral tane Mimi tane |
| Tt | ≤28 |
| CV | ≤10.0% |
| LoD | 400 Kopi/mL |
| Fa'apitoa | e leai se felafolafoaiga i le va o lenei pusa ma isi faʻamaʻi pipisi o le genitourinary tract e pei ole papillomavirus ituaiga tagata 16, ituaiga papillomavirus tagata 18, Herpes simplex virus type Ⅱ, Treponema pallidum, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Staphylococcus epidermis, Epidermis epidermis. Candida albicans, Trichomonas vaginalis, Lactobacillus crispatus, Adenovirus, Cytomegalovirus, Beta Streptococcus, Human immunodeficiency virus, Lactobacillus casei ma DNA genomic tagata, ma isi. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Polokalama PCR Taimi Moni ma BioRad CFX Opus 96 Faiga PCR Taimi Moni Faigofie Amp Fa'aola-Taimi Fluorescence Isothermal Detection System(HWTS-1600）. |
Galulue Galuega
Filifiliga 1.
Macro & Micro-Su'ega Fa'ata'ita'i Fa'ata'ita'iga Reagent (HWTS-3005-8). E tatau ona fa'ataunu'uina le fa'aputuga e tusa ma le IFU. Fa'aopoopo le DNA fa'ata'ita'i na maua mai i le fa'ata'ita'iga fa'asa'olotoga i totonu o le tali fa'amalo ma su'e sa'o le meafaigāluega, po'o fa'ata'ita'iga ua maua e tatau ona teu i le 2-8 ℃ mo le sili atu i le 24 itula.
Filifiliga 2.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). E tatau ona faʻatinoina le faʻaaogaina e tusa ai ma le IFU, ma o le volume elution fautuaina o le 80μL. Ole DNA fa'ata'ita'i na maua mai ile faiga fa'amaneta e fa'avevela ile 95°C mo le 3 minute ona ta'ele loa lea ole aisa mo le 2 minute. Fa'aopoopo le DNA fa'ata'ita'i ua mae'a fa'agaoioi i totonu o le tali fa'apolopolo ma fa'ata'ita'i le meafaigāluega po'o fa'ata'ita'iga fa'ata'ita'i e tatau ona teuina i lalo -18°C mo le le sili atu i le 4 masina. Ole numera ole fa'a'aisa fa'alua ma le liusua e le tatau ona sili atu ile 4 ta'amilosaga.
