Enterovirus Universal
Igoa o oloa
HWTS-EV001- Enterovirus Universal Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
O fa'ama'i lima-vae-gutu ose fa'ama'i pipisi e mafua mai ile enteroviruses (EV).I le taimi nei, 108 ituaiga o serotypes o enteroviruses ua maua, lea ua vaevaeina i ni vaega se fa: A, B, C ma D. Faatasi ai ma i latou, enterovirus EV71 ma CoxA16 o pathogens autu.Ole fa'ama'i e tele lava ina tupu i tamaiti i lalo ole 5 tausaga, ma e mafai ona mafua ai le herpes i lima, vae, gutu ma isi vaega.O se numera to'aitiiti o tamaiti o le a atia'e fa'alavelave e pei ole myocarditis, pulmonary edema, ma aseptic maningoencephalitis.
Auala
| FAM | EV RNA |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab,Herpes vai |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500Kopi/mL |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500/7500 Va'aiga Fa'atonu PCR Taimi Moni, QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fautuaina Pusa Aveesea: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor ( HWTS-3006B, HWTS-3006C), e tatau ona aveese faʻapitoa e tusa ai ma faatonuga.Ole fa'ata'ita'iga voluma o le 200 μL, o le fa'ata'ita'iga elution volume ole 80μL.
Filifiliga 2.
Pusa fa'atagaga fautuaina: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), e tatau ona fa'asa'o e tusa ai ma fa'atonuga.
Filifiliga3.
Pusa fa'atagaga fautuaina: QIAamp Viral RNA Mini Kit (52904) po'o le Nucleic Acid Extraction po'o le Purification Kit (YDP315-R), e tatau ona aveese mai e tusa ai ma faatonuga.Ole fa'ata'ita'iga voluma o le 140 μL, o le fa'ata'ita'iga elution volume ole 60μL.
