Valu ituaiga o siama o le manava
Igoa o le oloa
HWTS-RT184-Pusa e Iloa ai le Nucleic Acid mo Vairasi e Valu (Fluorescence PCR)
Fa'ama'i pipisi
O le faʻamaʻi pipisi o le manava o le ituaiga faʻamaʻi sili ona taatele lea o tagata, e mafai ona tupu i soʻo se itupa, matua ma itulagi, ma o se tasi lea o mafuaʻaga sili ona taua o le maʻi ma le oti i le lalolagi.[1]O siama fa'ama'i e masani ona a'afia i le manava e aofia ai le siama o le influenza A (IFV A), le siama o le influenza B (IFV B), le siama o le respiratory syncytial, adenovirus, human metapneumovirus, rhinovirus, le siama o le parainfluenza (I/II/III) ma le mycoplasma pneumoniae, ma isi.[2,3]E tutusa lava fa'ailoga ma fa'ailoga tau togafitiga e mafua mai i fa'ama'i pipisi o le manava, ae o fa'ama'i pipisi e mafua mai i siama eseese e eseese auala e togafitia ai, aafiaga fa'amalolo ma le a'afiaga o le fa'ama'i.[4,5]I le taimi nei, o auala autū e iloa ai siama o le manava e aofia ai: le vavaeʻeseina o siama, le iloa o antigen ma le iloa o nucleic acid. O lenei pusa e iloa ma faʻailoa ai ni viral nucleic acid patino i tagata taʻitoʻatasi e iai faʻailoga ma aʻafiaga o faʻamaʻi pipisi o le manava, faʻatasi ai ma isi faʻamaoniga faafomaʻi ma suʻesuʻega e fesoasoani ai i le suʻesuʻeina o faʻamaʻi pipisi o le manava.
Fa'atulagaga Fa'apitoa
| Teuina | 2-8℃ |
| Aso e teu ai | 12 masina |
| Ituaiga Fa'ata'ita'iga | Su'isu'i o le gutu; Su'isu'i o le isu |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 Kopi/mL |
| Fa'apitoa | Tali atu i isi siama: E leai se tali atu i isi siama i le va o le kit ma le siama Boca, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, human genomic DNA. Su'ega fa'alavelave: Filifili le mucin (60mg/mL), toto o le tagata (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride ma le 5% fa'asao (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), O taunu'uga na faʻaalia ai o mea faʻalavelave i le mea o loʻo i luga |
| Meafaigaluega Fa'aoga | E fa'atatau i le vaila'au e iloa ai le ituaiga I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Faiga PCR Taimi Moni, Faiga PCR Taimi Moni SLAN-96P (Hongshi Medical Technology Co., Ltd.), Faiga Iloa PCR Taimi Moni LineGene 9600 Plus (FQD-96A, tekinolosi Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), Faiga PCR Taimi Moni BioRad CFX96, Faiga PCR Taimi Moni BioRad CFX Opus 96. E fa'atatau i le vaila'au e iloa ai le ituaiga II: EudemonTM AIO800 (HWTS-EQ007) mai Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Tafega o Galuega
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e mafai ona fa'aaogaina fa'atasi ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ma le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (e mafai ona fa'aaogaina fa'atasi ma le EudemonTM AIO800 (HWTS-EQ007)) e le Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
O le aofaʻi o le faʻataʻitaʻiga na faʻaosoina e 200μL ma o le aofaʻi fautuaina o le faʻaosoina e 150μL.
