E valu Ituaiga Vairusi Manava
Igoa o oloa
HWTS-RT184-Ituaiga e valu o Vairusi Fa'amanava (Fluorescence PCR)
Epidemiology
O fa'ama'i o le manava o le fa'ama'i sili ona taatele o le tagata, e mafai ona tupu i so'o se itupa, tausaga ma le itulagi, ma o se tasi lea o mafua'aga sili ona taua o le ma'i ma le oti i le lalolagi.[1]. O fa'ama'i fa'ama'i masani fa'apitoa e aofia ai le influenza A virus (IFV A), influenza B virus (IFV B), respiratory syncytial virus, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) ma mycoplasma pneumoniae, ma isi.[2,3]. O fa'ailoga ma fa'ailoga e mafua mai i fa'ama'i o le manava e fai lava si tutusa, ae o le fa'ama'i e mafua mai i fa'ama'i 'ese'ese e eseese auala e togafitia ai, a'afiaga e fa'amālōlō ai ma fa'ama'i.[4,5]. I le taimi nei, o auala autu o suʻesuʻega suʻesuʻe o faʻamaʻi manava e aofia ai: faʻamaʻi faʻamaʻi, suʻesuʻega antigen ma suʻesuʻega nucleic acid. O lenei pusa e su'e ma fa'ailoa ai vaila'au nucleic fa'apitoa i tagata ta'ito'atasi o lo'o iai fa'ailoga ma fa'ailoga o fa'ama'i pipisi, fa'atasi ma isi fa'ai'uga o falema'i ma falesu'esu'e e fesoasoani ile su'esu'ega ole fa'ama'i pipisi ole manava.
Vaega Fa'ainisinia
| Teuina | 2-8 ℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 Kopi/mL |
| Fa'apitoa | Fa'asagaga fa'afeagai: E leai se fe'avea'i fa'asaga i le va o le pusa ma le virusi Boca, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, misela virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptoellacoccus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Klebsigenes Pneumonia Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Corynebaclicus DNA, Corynebaccillus human. Su'ega fa'alavelave: Filifili le mucin (60mg/mL), toto o le tagata (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride fa'atasi ma le 5% fa'asao (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), dexamethasone (20mg/mL) (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpiromL (20mg/mL), tomybramL (20mg/mL), mucinpirocin (20mg/mL). oseltamivir (60ng/mL), ribavirin (10mg/L), O faʻaiʻuga na faʻaalia ai o mea faʻalavelave i luga ole concentra |
| Meafaigaluega Faatatau | Fa'atatau i le ituaiga I su'esu'ega reagent: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Faiga PCR Taimi Moni, SLAN-96P Taimi Moni PCR Faiga (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Real Time CFX96), System BioRad CFX96. Opus 96 Polokalama PCR Taimi Moni. Fa'atatau i le ituaiga II su'esu'ega reagent: EudemonTM AIO800 (HWTS-EQ007) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Galulue Galuega
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ma le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (lea e mafai ona fa'aogaina e Euch)TM AIO800 (HWTS-EQ007)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
O le voluma fa'ata'ita'iga na maua mai o le 200μL ma o le voluma elution fautuaina o le 150μL.
