Chlamydia Trachomatis, Neisseria Gonorrhoeae ma Trichomonas vaginalis
Igoa o le oloa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae ma Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Fa'ama'i pipisi
O le Chlamydia trachomatis (CT) o se ituaiga o meaola ninii prokaryotic e na'o parasitic i totonu o sela eukaryotic. Ua vaevaeina le Chlamydia trachomatis i ni AK serotypes e tusa ai ma le metotia o le serotype. O fa'ama'i pipisi o le urogenital tract e mafua tele lava i le trachoma biological variant DK serotypes, ma o tane e masani ona fa'aalia o le urethritis, lea e mafai ona fa'amalolo e aunoa ma se togafitiga, ae o le tele o ia mea e tumau, fa'ateteleina i nisi taimi, ma e mafai ona tu'ufa'atasia ma le epididymitis, proctitis, ma isi mea fa'apena.
Alavai
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Vaginitis Trichomonal |
| VIC/HEX | Pulega i Totonu |
Fa'atulagaga Fa'apitoa
| Teuina | -18℃ |
| Aso e teu ai | 12 masina |
| Ituaiga Fa'ata'ita'iga | O le su'isu'i o le ua o fafine,Su'isu'i o le measā fafine,Suavai o le urethral tane |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Kopi/mL |
| Fa'apitoa | E leai se fesootaʻiga ma isi siama faʻamaʻi pipisi i feusuaiga (STD) i tua atu o le vaega e mafai ona iloa i le pusa suʻega, e pei o le Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, ma isi. |
| Meafaigaluega Fa'aoga | Fa'aogaina o le Biosystems 7500 Real-Time PCR System Fa'aogaina o Biosystems 7500 Faiga PCR Vave i le Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Polokalame PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni Polokalama Iloa PCR Taimi Moni a le LineGene 9600 Plus MA-6000 Fa'ata'amilosaga Fa'avevela Fa'atatau i le Taimi Moni BioRad CFX96 Polokalame PCR Taimi Moni BioRad CFX Opus 96 Polokalame PCR Taimi Moni |
Tafega o Galuega
Filifiliga 1.
Mea fa'aopoopo e fautuaina mo le fa'amamāina: Fa'asusu le 1mL o le fa'ata'ita'iga e fa'ata'ita'i i totonu o se paipa centrifuge e leai se DNase/RNase e 1.5mL, fa'amamā i le 12000rpm mo le 3 minute, lafoa'i le supnatant ma taofi le precipitate. Fa'aopopo le 200µL o le normal saline i le precipitate e toe fa'asusu ai. Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (lea e mafai ona fa'aaogaina fa'atasi ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) mai Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faia le fa'amamāina e tusa ai ma fa'atonuga mo le fa'aoga. O le tele o le fa'ata'ita'iga na fa'amamāina e 200µL, ma o le tele o le elution e fautuaina e 80µL.
Filifiliga 2.
Mea fa'aoso e fautuaina: Nucleic Acid Extraction po'o le Purification Kit (YDP302). E tatau ona faia le fa'aoso e tusa ai ma fa'atonuga mo le fa'aoga. O le tele fautuaina o le elution e 80µL.
