Chlamydia Trachomatis, Neisseria Gonorrhoeae ma Trichomonas vaginalis
Igoa o oloa
HWTS-UR041 Chlamydia Trachomatis, Neisseria Gonorrhoeae ma Trichomonas vaginalis Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Chlamydia trachomatis (CT) o se ituaiga o microorganism prokaryotic e matua faʻamaʻi i sela eukaryotic.Chlamydia trachomatis ua vaevaeina i AK serotypes e tusa ai ma le auala serotype.O faʻamaʻi pipisi o le urogenital e tele lava ina mafua mai i le trachoma biological variant DK serotypes, ma o tane e tele lava ina faʻaalia o le urethritis, lea e mafai ona faʻamalolo e aunoa ma se togafitiga, ae o le tele o latou e faʻamaʻi, faʻalavelave faʻafuaseʻi, ma e mafai ona tuʻufaʻatasia ma epididymitis, proctitis, ma isi.
Auala
| FAM | Chlamydia trachomatis |
| ROX | Neisseria gonorrhoeae |
| CY5 | Trichomonal vaginitis |
| VIC/HEX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | -18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Su'e fa'atama'ita'i,Fua fa'afafine,Usu urethral tane |
| Ct | ≤38 |
| CV | <5% |
| LoD | 400Kopi/mL |
| Fa'apitoa | E leai se feso'ota'iga fa'atasi ma isi fa'ama'i pipisi STD i fafo atu o le va'aiga o le pusa su'ega, e pei o Treponema pallidum, Mycoplasma hominis, Mycoplasma genitalium, Herpes simplex virus type 1, Herpes simplex virus type 2, Candida albicans, ma isi. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fautuaina le fa'aaogaina o le fa'aputuga: Pipette 1mL o le fa'ata'ita'iga e fa'ata'ita'i ile 1.5mL ole DNase/RNase-free centrifuge paipa, centrifuge i le 12000rpm mo le 3 minute, lafoa'i le supernatant ma fa'atumauina le pa.Fa'aopoopo le 200µL o le masima masani i le fa'afefete e toe fa'atumauina.Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (lea e mafai ona faʻaaogaina ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faia le suʻega e tusa ai ma faatonuga mo le faʻaaogaina.O le voluma fa'ata'ita'iga na maua mai o le 200µL, ma o le voluma fa'ata'ita'i fautuaina o le 80μL.
Filifiliga 2.
Fautuaina le fa'asusuina o mea fa'apipi'i: Nucleic Acid Extraction po'o le Purification Kit (YDP302).E tatau ona faia sa'o le fa'aaogaina e tusa ai ma fa'atonuga mo le fa'aoga.Ole voluma fa'amama fa'atonu e 80µL.
