Carbapenem tetee o Gene (KPC / NDM / OXA 48 / OXA 23 / VIM / IT)
Igoa o oloa
HWTS-OT045 Carbapenem Faʻatusatusaina Gene (KPC / NDM / OXA 48 / OXA 23 / VIM (FOMOCRESSCECY PRD)
Leitio
Carbapenem antibiotics o AyyPical β-lactam antibiotics ma le lautele o le aspibacterlial specrum ma sili ona malosi gaioiga. Ona o lona tulaga maualuga i le β-lactamase ma maualalo oona, ua avea ma se tasi o le sili ona taua fua faatatau o fualaau faasaina. O Carbapenems e sili ona mautu i luga o le plasmid-spectrad β-spectrum β (esbls), chromosomes cephalocolassis (Ampc-netmid
Auala
| PCR-palue 1 | PCR-Mifiloi 2 | |
| Oge | O lona uiga | Vim |
| Vic / HEX | Pusa i totonu | Pusa i totonu |
| Cy5 | Ni | KPC |
| Rox | Oxa48
| Oxa23 |
Tulaga faapitoa
| Teuina | ≤ - 18 ℃ |
| Fata-ola | 12 masina |
| Primetien ituaiga | Sputmu, mama kolone, o le rectal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lote | 103Cfu / ml |
| Faʻapitoa | A) O le pusa e iloa ai faʻamatalaga faʻapitoa a le tagata o le tagata, ma o iʻuga e fetaui i manaʻoga o mea tutusa. b) O iʻuga o le satauro-fuafua suʻega faʻaalia ai o lenei pusa e leai se koluseina o loʻo iai ma isi manava, o le araesocacs asytoni, Haemophils Faʻalauiloa, Acinietobacter Junii, Acinietobacter Haemonos, Eschesotory Frees-Fortures, CHANDOCUCS, CHORMOCUS, CHANMOCOMUS, Mekala, na manogi, shv, tem, ma isi mea c) anti-inference: mucin, Minocycline, Nuuese, IMandamycin, Chowoperaxin, o le Cheavopancin e leai se faalavelave i le iloa o Carbapenem tetee o Gene KPC, NDm, Oxa48, Oxa23, vim, ma tulaga. |
| Talosaga talafeagai | Faʻaaogaina Biosyystem 7500 Twy-Time PCR polokalama Aofai o le numera numera-taimi Slan-96p moni-taimi pcr polokalama (Hentshi Medical Technical Co., Ltd.) Malamalama clucklecle®480 moni-taimi pcr system Linegene 9600 faaopoopo le moni-taimi pcr maua system system (FQD-96A,HaszhouBioder tekonolosi) Ma-6000 moni-taimi le aofaʻi o le malae taʻavale taʻavale Biorad cfx96 moni-taimi pcr system Biorad CFX OPSS 96 moni-taimi pcr system |
Galuega tafe
Filifiliga 1.
Fautuaina le faʻaaogaina o le faʻaaogaina: Macro & Micro-Suʻega General Pran's DNA / RNA-Hwts-3019-50, hwts-3019-32, Hwts-3019-48, Hwts-3019-96) (lea e mafai ona faʻaaogaina ma Macro & Micro-Suʻega otometi Nuckic Acad Expracractor (HWTS o le thalls o loo i ai. O Laʻasaga Mulimuli mai e tatau ona mulimuli i faʻatonuga mo le tuʻufaʻatasia, ma le faʻailoga fautuaina o100μl.
Filifiliga 2.
Fautuaina le faʻaaogaina o le faʻaaogaina: Nuckoric Acad Extraction poʻo le faʻamamaina , ma luluina seiloga e faʻamalolo le 'aʻe i le' au Faʻaaoga le RNANE / DNASE FUA FUA MO LE LAUGA, Ma o le elvamed Tree Malosiaga o le 100μl.
Filifiliga 3.
Fautuaina le faʻaaogaina o le faʻaaogaina: Macro & Micro-Suʻega faʻataʻitaʻiga na faʻatagaina O le Sputum faʻataʻitaʻiga e tatau ona fufuluina i le faʻaopopoina o le 1ml o le masani masima i luga o le sili ona taua o le thalls o loʻo taʻua, centat, ma le supernat ua lafoaia (gentatant Mo le mama faaumini ma factul swab, faʻaopopo le 50μl o le faʻataʻitaʻiga faʻasaʻoloto saʻo i luga o le sili ona taua o le trlls orsitable, ma o ni sitepu e tatau ona toe faʻafoʻi e tusa ma le faʻatonuga.
