Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP)
Igoa o oloa
HWTS-OT045 Carbapenem Resistance Gene (KPC/NDM/OXA 48/OXA 23/VIM/IMP) Pusa Su'esu'ega (Fluorescence PCR)
Epidemiology
O vailaʻau faʻamaʻi Carbapenem o vailaʻau faʻamaʻi β-lactam faʻatasi ma le lautele o alaleo faʻamaʻi faʻamaʻi ma sili ona malosi gaioiga faʻamaʻi.Ona o lona mautu i le β-lactamase ma le maualalo o le oona, ua avea ma se tasi o vailaʻau antibacterial sili ona taua mo le togafitia o faʻamaʻi pipisi ogaoga.Carbapenems e matua mautu i plasmid-mediated extended-spectrum β-lactamases (ESBLs), chromosomes, ma plasmid-mediated cephalosporinases (AmpC enzymes).
Auala
| PCR-Faafefiloi 1 | PCR-Mix 2 | |
| FAM | IMP | VIM |
| VIC/HEX | Pulea i totonu | Pulea i totonu |
| CY5 | NDM | KPC |
| ROX | OXA48
| OXA23 |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Sputum, colonies mama, swab rectal |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 103CFU/mL |
| Fa'apitoa | a) E iloa e le pusa faʻamatalaga le lelei a le kamupani, ma o taunuuga e fetaui ma manaʻoga o faʻamatalaga tutusa. b) O faʻaiʻuga o le suʻega faʻasolosolo e faʻaalia ai o lenei pusa e leai se tali faʻafefe ma isi faʻamaʻi pipisi, e pei ole Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenza, Acinetobacter influenza. junii, Acinetobacter haemolyticus, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory adenovirus, Enterococcus, poʻo faʻataʻitaʻiga o loʻo iai isi vailaʻau faʻamaʻi faʻamaʻi CTX, mecA, SME, SHV, TEM, ma isi. c) Faʻalavelave faʻalavelave: Mucin, Minocycline, Gentamicin, Clindamycin, Imipenem, Cefoperazone, Meropenem, Ciprofloxacin Hydrochloride, Levofloxacin, Clavulanic acid, Roxithromycin ua filifilia mo suʻega faʻalavelave, ma o faʻaiʻuga e faʻaalia ai e leai se faʻalavelave faʻalavelave ua taʻua i luga. i le suʻesuʻeina o kenera tetee o le carbapenem KPC, NDM, OXA48, OXA23, VIM, ma le IMP. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni (Hongshi Medical Technology Co., Ltd. ) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A,HangzhouBioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fa'atonuga ole su'ega: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (lea e mafai ona faʻaaogaina i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Faʻaopoopo le 200μL o le saline masani ile o le thallus precipitate.O laasaga mulimuli ane e tatau ona mulimulita'i i fa'atonuga mo le su'eina, ma o le voluma elution fautuaina e100μL.
Filifiliga 2.
Fautuaina le fa'aaogaina o le fa'aputuina: Nucleic Acid Extraction po'o le Purification Reagent (YDP302) e Tiangen Biotech (Beijing) Co., Ltd. E tatau ona amata le fa'aputuga e tusa ma le la'asaga 2 o le fa'atonuga mo le fa'aoga (fa'aopoopo le 200μL o le pa puipui GA i le thallus precipitate. , ma lulu seʻia oʻo ina taofi atoa le thallus).Fa'aaoga le RNase/DNase vai e leai se totogi mo le fa'amama, ma le fa'atonuga o le voluma e 100μL.
Filifiliga 3.
Fautuaina le fa'asusuina o mea fa'apipi'i: Macro & Micro-Su'ega Fa'ata'ita'i Fa'ata'ita'iga Reagent.E mana'omia ona fufulu le fa'ata'ita'iga o le sputum e ala i le fa'aopoopoina o le 1mL o le masima masani i le fa'afefeteina o lo'o ta'ua i luga, fa'amama i le 13000r/min mo le 5 minute, ma lafoa'i le supernatant (tausia le 10-20μL o le supernatant).Mo colony mama ma rectal swab, faaopoopo 50μL o faataitaiga faamatuu atu reagent tuusao i le ua taua i luga togafitia thallus precipitate, ma le laasaga mulimuli e tatau ona aveesea e tusa ai ma faatonuga mo le faaaogaina.
