4 Ituaiga Vairusi Manava Nucleic Acid

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻaaogaina mo le suʻesuʻeina lelei o le SARS-CoV-2, faʻamaʻi o le influenza A, faʻamaʻi B ma le respiratory syncytial virus nucleic acids i faʻataʻitaʻiga swab oropharyngeal tagata.


Fa'amatalaga Oloa

Faailoga o oloa

Igoa o oloa

HWTS-RT075-Fa'a'aisa-fa'amago 4 Ituaiga Fa'ama'i Vairusi Pusa Su'esu'ega Nucleic Acid (Fluorescence PCR)

Epidemiology

O le fa'ama'i o le Corona Virus 2019, e ta'ua o le "COVID-19", e fa'atatau i le niumonia e mafua mai i fa'ama'i pipisi o le SARS-CoV-2.O le SARS-CoV-2 o se coronavirus e patino ile ituaiga β.O le COVID-19 ose fa'ama'i fa'ama'i pipisi, ma e masani ona a'afia le faitau aofa'i.I le taimi nei, o le mafuaʻaga o faʻamaʻi pipisi e masani lava o tagata mamaʻi e aʻafia i le SARS-CoV-2, ma o tagata aʻafia e leai ni faʻamaʻi e mafai foi ona avea ma puna o faʻamaʻi.Faʻavae i luga o suʻesuʻega faʻamaʻi o loʻo iai nei, o le taimi faʻamaʻi e 1-14 aso, tele 3-7 aso.O le fiva, tale mago ma le vaivai o fa'aaliga autu ia.O nai tagata mama'i na iai fa'ailoga e pei o le pipii o le isu, tafe le isu, tiga fa'a'i, myalgia ma le manava, ma isi.

O le fulū, e masani ona ta'ua o le "flu", ose fa'ama'i fa'ama'i fa'ama'i pipisi e mafua mai i le fulū.E maualuga tele le pipisi.E masani lava ona feavea'i e ala i le tale ma le mafatua.E masani ona oso i le tautotogo ma le taumalulu.O fa'ama'i fa'ama'i e vaevaeina i le fulū A (IFV A), fa'ama'i B (IFV B), ma le fa'ama'i C (IFV C) e tolu ituaiga, e a'afia uma i siama pipii, e mafua ai fa'ama'i o le tagata ma'i mo siama A ma le B, e tasi lava. -pa'ia, RNA virusi vaega.Ole fa'ama'i ole Influenza A ose fa'ama'i pipisi tele ile manava, e aofia ai le H1N1, H3N2 ma isi fa'ama'i laiti, ia e faigofie ona suia ma fa'ama'i i le lalolagi atoa.O le "Shift" e faasino i le suiga o le influenza A virus, e mafua ai ona aliaʻe se virus fou "subtype".O fa'ama'i fa'ama'i fa'ama'i B ua vaevaeina i gafa e lua, Yamagata ma Vitoria.O le fa'ama'i o le Influenza B e na'o le antigenic drift, ma e 'alo'ese mai le mata'ituina ma le fa'ate'aina a le tagata e ala i ona suiga.Ae ui i lea, o le saoasaoa o le evolusione o le influenza B virus e telegese nai lo le virus influenza A tagata.Ole fa'ama'i ole Influenza B e mafai fo'i ona fa'atupu fa'ama'i ile manava ole tagata ma ta'ita'i atu ai ile fa'ama'i.

Respiratory syncytial virus (RSV) o se siama RNA, e patino i le aiga paramyxoviridae.E feavea'i e ala i matāua o le ea ma feso'ota'i vavalalata ma o le fa'ama'i autu lea o fa'ama'i pipisi o le manava maualalo i pepe.O pepe e a'afia ile RSV e ono atia'e le bronchiolitis tuga ma le niumonia, lea e feso'ota'i ma le sela i tamaiti.O pepe e iai auga ogaoga, e aofia ai le fiva maualuga, rhinitis, pharyngitis ma laryngitis, ona sosoo ai lea ma le bronchiolitis ma le niumonia.O nai tamaiti mama'i e mafai ona faigata ile otitis media, pleurisy ma myocarditis, ma isi.

Auala

FAM SARS-CoV-2
VIC(HEX) RSV
CY5 IFV A
ROX IFV B
NED

Pulea i totonu

Vaega Fa'atekinisi

Teuina 2-8°C
Fa'asa-ola 12 masina
Ituaiga Fa'ata'ita'iga Oropharyngeal swab
Ct ≤38
LoD SARS-CoV-2: 150Kopi/mL

Fa'ama'i o le Influenza A/Varusi Fulu'i B/ Fa'ama'i fa'atasi ma le manava: 300Kopi/mL

Fa'apitoa E leai se feso'ota'iga fa'afeagai ma tagata coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus tagata, enterovirus A, B, C, D, pulmonary virus, epstein-barr virus, misle virus, cytomegalo virus tagata, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, asu aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci ma cryptococcus faatoa fanau mai ma tagata genomic acid nucleic.
Meafaigaluega Faatatau Fa'aogaina Biosystems 7500 Taimi Moni PCR System, Fa'aogaina Biosystems 7500 Va'ai Va'aiga Fa'atonu PCR Fa'atonu, QuantStudio®5 Taimi Moni PCR Systems

Galulue Galuega

Filifiliga 1.

Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).

Filifiliga 2.

Fautuaina le fa'aputuina o mea fa'apipi'i: Nucleic Acid Extraction po'o le Purification Reagent(YDP302) e Tiangen Biotech(Beijing) Co.,Ltd.


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