17 Ituaiga HPV (16/18/6/11/44 Tu'aiga)
Igoa o oloa
HWTS-CC015 17 Ituaiga o Human Papillomavirus (16/18/6/11/44 Tu'aiga) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
O le kanesa o le faa'au'auna o se tasi lea o tuma ma'i e sili ona taatele i le auga fanau tama'ita'i. Ua fa'aalia o fa'ama'i pipisi o le HPV ma le tele o fa'ama'i ose tasi lea o mafua'aga tele o le kanesa fa'a'a'au. I le taimi nei o lo'o i ai pea le le lava o togafitiga lelei e talia lautele mo le kanesa fa'a'a'au e mafua mai i le HPV. O le mea lea, o le vave su'esu'eina ma le puipuia o fa'ama'i fa'ama'i fa'aa'au e mafua mai i le HPV o ki ia e puipuia ai le kanesa fa'aa'au. O le fa'atuina o su'esu'ega fa'apitoa, fa'apitoa ma fa'avavevave mo fa'ama'i fa'ama'i e taua tele mo su'esu'ega fa'afoma'i ole kanesa fa'a'a'au.
Auala
| PCR-Mix1 | FAM | 18 |
| VIC/HEX | 16 | |
| ROX | 31,33,35,39,45,51,52,56,58,59,66,68 | |
| CY5 | Pulea totonu |
| PCR-Mix2 | FAM | 6 |
| VIC/HEX | 11 | |
| ROX | 44 | |
| CY5 | Pulea totonu |
Vaega Fa'ainisinia
| Teuina | -18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Fa'ata'ita'iga o le mimi, fa'ata'ita'i su'ega tama'ita'i fa'ata'ita'i, fa'ata'ita'iga su'ega fa'atama'ita'i |
| Ct | ≤28 |
| LoD | 300Kopi/mL |
| Fa'apitoa | E leai se feso'ota'iga fa'afeagai ma Ureaplasma urealyticum, Chlamydia trachomatis o le vaega fanau, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mold, Gardnerella ma isi ituaiga HPV e le o aofia i le pusa. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (0H6C Acid Extractor) HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Faʻaopoopo le 200μL o le saline masani e toe faʻaleleia ai le pellet i le laasaga 2.1, ona tatau ai lea ona faʻatautaia le suʻega e tusa ai ma faʻatonuga mo le faʻaogaina o lenei reagent. Ole fua ole elution fautuaina ole 80μL.
Fautuaina le fa'aaogaina o mea fa'apipi'i: QIAamp DNA Mini Kit (51304) po'o le Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Faʻaopoopo le 200μL o le saline masani e toe faʻaleleia ai le pellet i le laasaga 2.1, ona tatau ai lea ona faʻatautaia le aveeseina e tusa ai ma faʻatonuga mo le faʻaogaina o lenei mea faʻapipiʻi. O le faʻataʻitaʻiga faʻataʻitaʻiga voluma o faʻataʻitaʻiga e 200μL uma, ma o le voluma elution fautuaina o le 100μL.
Fa'atonuga fa'ameamea: Fa'ata'ita'i Fa'ata'ita'i Fa'ata'ita'i Fa'ata'ita'i (HWTS-3005-8I, HWTS-3005-8J, HWTS-3005-8K, HWTS-3005-8L). Faʻaopoopo le 200μL o faʻataʻitaʻiga faʻasaʻo faʻamalo e toe faʻaleleia le pellet i le laasaga 2.1, ona tatau lea ona faʻatautaia le faʻaaogaina e tusa ai ma faʻatonuga mo le faʻaogaina o lenei mea faʻapipiʻi.
