14 Ituaiga o Manu'a Manava Tuufaatasi
Igoa o oloa
HWTS-RT159B 14 Ituaiga Fa'ama'i Manava Tuufaatasi Pusa Su'esu'ega Nucleic Acid (Fluorescence PCR)
Epidemiology
O fa'ama'i fa'ama'i o le fa'ama'i taatele lea i tagata, e mafai ona tupu i so'o se itupa, tausaga ma le itulagi. O se tasi lea o mafuaʻaga autu o le maʻi ma le oti i le faitau aofaʻi o le lalolagi[1]. O faʻamaʻi pipisi masani e aofia ai le coronavirus fou, Influenza A virus, Influenza B virus, Respiratory syncytial virus, Adenovirus, human metapneumovirus, rhinovirus, Parainfluenza virus type I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, ma Streptococcus pneumoniae, ma isi.[2,3].
Auala
| Lelei Tulaga | Igoa Fofo Tali | Patogen e Su'eina |
| 1 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Mix B | Adv, hMPV, MP, Cpn |
| 3 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Mix D | CoV, EV, SP, pulea totonu |
| 5 | Master Mix A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Mix B | Adv, hMPV, MP, Cpn |
| 7 | Master Mix C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Mix D | CoV, EV, SP, pulea totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab, Nasopharyngeal swab |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200Kopi/mL |
| Fa'apitoa | O fa'ai'uga o su'esu'ega fa'afeagai na fa'ailoa mai ai e leai se fa'alavelave fa'asaga i le va o lenei pusa ma Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilalis, Morale pneumophilalist. Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Corstriatum cepa, No. Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii ma tagata genomic nuclei. |
| Meafaigaluega Faatatau | SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.)Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Polokalama PCR Taimi Moni, BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Galulue Galuega
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. O le volume sample µL. O laasaga mulimuli e tatau ona faia e tusa ai ma faʻatonuga mo le faʻaogaina o lenei mea faʻapipiʻi. Ole voluma elution fautuaina ole80µL.
