14 ituaiga o siama o le manava ua tuufaatasia
Igoa o le oloa
HWTS-RT159B 14 Itūaiga o Ma'i Fa'ama'i Manava Tu'ufa'atasi Nucleic Acid Detection Kit (Fluorescence PCR)
Fa'ama'i pipisi
O le faʻamaʻi pipisi o le manava o le faʻamaʻi sili ona taatele i tagata, e mafai ona tupu i soʻo se itupa, matua ma itulagi. O se tasi lea o mafuaʻaga autū o le maʻi ma le oti i le faitau aofaʻi i le lalolagi atoa.[1]O siama masani o le manava e aofia ai le coronavirus fou, le siama o le Influenza A, le siama o le Influenza B, le siama o le Respiratory syncytial, Adenovirus, le human metapneumovirus, le rhinovirus, le siama o le Parainfluenza type I/II/III/IV, le Bocavirus, le Enterovirus, le Coronavirus, le Mycoplasma pneumoniae, le Chlamydia pneumoniae, ma le Streptococcus pneumoniae, ma isi.[2,3].
Alavai
| Tulaga o le Vaieli | Igoa o le Vai Fa'afoma'i | O siama e tatau ona iloa |
| 1 | Fa'afefiloi Sili A | SARS-CoV-2, IFV A, IFV B |
| 2 | Fa'afefiloi Sili B | Fautua, hMPV, MP, Cpn |
| 3 | Fa'afefiloi Sili C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Fa'afefiloi Sili D | CoV, EV, SP, pulega i totonu |
| 5 | Fa'afefiloi Sili A | SARS-CoV-2, IFV A, IFV B |
| 6 | Fa'afefiloi Sili B | Fautua, hMPV, MP, Cpn |
| 7 | Fa'afefiloi Sili C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Fa'afefiloi Sili D | CoV, EV, SP, pulega i totonu |
Fa'atulagaga Fa'apitoa
| Teuina | ≤-18℃ |
| Aso e teu ai | 9 masina |
| Ituaiga Fa'ata'ita'iga | Su'isu'i o le gutu/oropharyngeal、Su'isu'i o le gutu/oropharyngeal |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 Kopi/mL |
| Fa'apitoa | O taunuuga o le suega cross-reactivity na faaalia ai e leai se cross reaction i le va o lenei kit ma le Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia. mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii ma siama nucleic genomic a le tagata. |
| Meafaigaluega Fa'aoga | SLAN-96P Polokalame PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.)Fa'aogaina o Biosystems 7500 Faiga PCR Taimi Moni Fa'aogaina o Biosystems 7500 Faiga PCR Vave i le Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni Polokalama Iloa o le PCR i le Taimi Moni a le LineGene 9600 Plus (FQD-96A, tekinolosi a Hangzhou Bioer) MA-6000 Fa'ata'amilosaga Fa'avevela Fa'atatau i le Taimi Moni (Suzhou Molarray Co., Ltd.) BioRad CFX96 Faiga PCR Taimi Moni, BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Tafega o Galuega
Mea fa'aola e fautuaina mo le fa'a'esea: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e mafai ona fa'aaogaina fa'atasi ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ010)) mai Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. O le aofa'i o le fa'ata'ita'iga e fa'a'esea e 200µL. O la'asaga e sosoo ai e tatau ona faia e tusa ai ma fa'atonuga mo le fa'aaogaina o lenei mea fa'aola. O le aofa'i fautuaina mo le fa'a'esea e80µL.
