Enterococcus e tetee i Vancomycin ma Gene e tetee i Vailaau
Igoa o oloa
HWTS-OT090-Vancomycin-tetetete Enterococcus ma Fualaau Fa'asaina Gene Su'esu'ega Pusa (Fluorescence PCR)
Epidemiology
Tete'e i Fualaau Fa'asaina o lo'o ta'ua fo'i o le tete'e o vaila'au, e fa'atatau i le tete'e atu o siama i le gaioiga o vaila'au fa'ama'i. O le taimi lava e tula'i mai ai le tete'e atu i vaila'au, o le a matua fa'aitiitia le aafiaga o le chemotherapy o vaila'au. Tete'e i Fualaau fa'asaina ua vaevaeina i totonu o le tete'e ma maua tete'e. O le tete'e i totonu e fuafua e kenera chromosomal siama, pasi mai lea tupulaga i lea tupulaga, ma o le a le suia. O le teteʻe maua e mafua ona o le mea moni e faapea pe a uma ona faʻafesoʻotaʻi ma vailaʻau faʻamaʻi, e suia e siama a latou lava auala metabolic ina ia le fasiotia i latou e vailaau faʻamaʻi.
Ole vancomycin resistance genes VanA ma VanB e maua mai fualaau fa'asaina, e aofia ai le VanA o loʻo faʻaalia le maualuga o le tetee i le vancomycin ma le teicoplanin, VanB o loʻo faʻaalia tulaga eseese o le tetee i le vancomycin, ma e maaleale ile teicoplanin. Vancomycin e masani ona faʻaaogaina ile falemaʻi e togafitia ai faʻamaʻi pipisi o le Gram-positive, ae ona o le tulaʻi mai o le vancomycin-resistant enterococci (VRE), aemaise lava enterococcus faecalis ma enterococcus faecium, e sili atu nai lo le 90%, ua aumaia ai ni luʻitau fou fou i togafitiga falemaʻi. I le taimi nei, e leai se vailaʻau faʻamaʻi faʻamaʻi faʻapitoa mo togafitiga o le VRE. E le gata i lea, e mafai fo'i e le VRE ona tu'uina atu kenera e tete'e atu i vaila'au i isi enterococci po'o isi siama Gram-positive.
Auala
FAM | Vancomycin-resistant enterococci (VRE): Enterococcus faecalis ma Enterococcus faecium |
VIC/HEX | Pulea i totonu |
CY5 | vancomycin resistance gene VanB |
ROX | vancomycin resistance gene VanA |
Vaega Fa'ainisinia
Teuina | Suavai: ≤-18℃ |
Fa'asa-ola | 12 masina |
Ituaiga Fa'ata'ita'iga | sputum, toto, urine po'o kolone mama |
CV | ≤5.0% |
Ct | ≤36 |
LoD | 103CFU/mL |
Fa'apitoa | E leai se fesoʻotaʻiga faʻatasi ma isi faʻamaʻi pipisi e pei o le klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. coli, epilepsy, legionoplasticus, a. pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, poʻo faʻataʻitaʻiga o loʻo i ai isi genes e faʻasalaina fualaau CTX, mecA, SME, Samples of SHV ma TEM. |
Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga |
Galulue Galuega
Reagents fa'atonuina: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).