SARS-CoV-2 Eseesega
Igoa o oloa
HWTS-RT072A-SARS-CoV-2 Pusa Su'esu'ega Eseese (Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
O le tala fou o le coronavirus (SARS-CoV-2) ua sosolo i se fua tele i le lalolagi atoa.I le faagasologa o le faʻasalalauga, o suiga fou e tupu i taimi uma, e mafua ai suiga fou.O lenei oloa e masani ona faʻaaogaina mo le suʻesuʻeina fesoasoani ma le eseesega o mataupu e fesoʻotaʻi ma faʻamaʻi pe a maeʻa le faʻasalalau tele o faʻamaʻi o le Alpha, Beta, Gamma, Delta ma Omicron mutant talu mai Tesema 2020.
Auala
FAM | N501Y, HV69-70del |
CY5 | 211-212del, K417N |
VIC(HEX) | E484K, Pulea totonu |
ROX | P681H, L452R |
Vaega Fa'atekinisi
Teuina | ≤-18℃ I le pogisa |
Fa'asa-ola | 9 masina |
Ituaiga Fa'ata'ita'iga | fa'asu'u nasopharyngeal, su'ega oropharyngeal |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000Kopi/mL |
Fa'apitoa | E leai se felafolafoaʻiga faʻatasi ma tagata coronavirus SARS-CoV ma isi faʻamaʻi masani. |
Meafaigaluega talafeagai: | QuantStudio™5 Faiga PCR Taimi Moni SLAN ®-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Filifiliga 2.
Fautuaina le fa'aputuina o mea fa'apipi'i: Nucleic Acid Extraction po'o le Purification Reagent(YDP302) e Tiangen Biotech(Beijing) Co.,Ltd.