Fa'atasiga o siama o le manava
Fa'amatalaga Tu'ufa'atasi o Fa'ama'i Manava:
Igoa o le oloa
HWTS-RT050-Ono Ituaiga o le Pusa e Iloa ai le Pathogen Nucleic Acid mo le Manava(PCR o le Fluorescence)
Fa'ama'i pipisi
O le fulū, e masani ona ta'ua o le 'fulū', o se fa'ama'i pipisi fa'afuase'i o le mānava e mafua mai i le siama o le fulū, lea e matua pipisi lava ma e masani ona pipisi e ala i le tale ma le mafatua.
O le siama Respiratory syncytial (RSV) o se siama RNA e aʻafia i le aiga o paramyxoviridae.
O le Human adenovirus (HAdV) o se siama DNA e lua ona fusi e leai se envelope. E le itiiti ifo ma le 90 genotypes ua maua, e mafai ona vaevaeina i le 7 subgenera AG.
O le Human rhinovirus (HRV) o se tasi o siama o le aiga Picornaviridae ma le ituaiga Enterovirus.
O le Mycoplasma pneumoniae (MP) o se siama fa'ama'i e iai le lapopo'a i le va o siama ma siama.
Alavai
| Alavai | PCR-Fefiloi A | PCR-Fefiloi B |
| Auala o le FAM | IFV A | HAdV |
| Alavai VIC/HEX | HRV | IFV B |
| Alaata CY5 | RSV | MP |
| Ala o le ROX | Pulega i Totonu | Pulega i Totonu |
Fa'atulagaga Fa'apitoa
| Teuina | -18℃ |
| Aso e teu ai | 12 masina |
| Ituaiga Fa'ata'ita'iga | Su'isu'i o le gutu (oropharyngeal swab) |
| Ct | ≤35 |
| LoD | 500 Kopi/mL |
| Fa'apitoa | 1.O taunuuga o le suega o le cross-reactivity na faaalia ai e leai se cross reaction i le va o le kit ma le human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, Parainfluenza virus types 1, 2, ma le 3, Chlamydia pneumoniae, human metapneumovirus, Enterovirus A, B, C, D, Epstein-Barr virus, Measles virus, human cytomegalovirus, Rotavirus, Norovirus, Mumps virus, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans ma human genomic nucleic acids. 2.Malosiaga e tete'e atu ai i fa'alavelave: Mucin (60mg/mL), 10% (v/v) toto o le tagata, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (fa'atasi ai ma mea fa'asao) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), ma le tobramycin (0.6mg/mL) na filifilia mo le suega fa'alavelave, ma o taunuuga na fa'aalia ai o mea fa'alavelave i le maualuga o lo'o i luga e leai se tali fa'alavelave i taunuuga o le suega o siama. |
| Meafaigaluega Fa'aoga | Fa'aogaina o Biosystems 7500 Faiga PCR Taimi Moni Fa'aogaina o Biosystems 7500 Faiga PCR Vave i le Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Polokalame PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni Polokalama Iloa PCR Taimi Moni a le LineGene 9600 Plus MA-6000 Fa'ata'amilosaga Fa'avevela Fa'atatau i le Taimi Moni BioRad CFX96 Faiga PCR Taimi Moni, BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Vaifofo PCR Atoa
Ata o fa'amatalaga o oloa:
Taiala o Oloa e Fesootai:
A o tau fa'atauva'a, matou te talitonu o le a e su'eina mea uma e mafai ona sili atu nai lo matou. E mafai ona matou fai atu ma le mautinoa atoatoa, mo le maualuga o le tulaga lelei i ia tau, o matou o le tulaga maualalo mo Respiratory Pathogens Combined, O le a tu'uina atu le oloa i le lalolagi atoa, e pei o: Sevilla, Europa, Mauritius. Afai e te fiafia i so'o se tasi o a matou oloa pe a uma ona e va'ai i la matou lisi o oloa, fa'amolemole fa'afeso'ota'i matou mo ni fesili. E mafai ona e lafoina mai imeli ma fa'afeso'ota'i matou mo ni feutagaiga ma o le a matou tali atu ia te oe i se taimi vave e mafai ai. Afai e talafeagai, e mafai ona e mauaina la matou tuatusi i la matou 'upega tafa'ilagi ma asiasi mai i la matou pisinisi. pe fa'amatalaga fa'aopoopo e uiga i a matou oloa na'o oe. Matou te sauni e fausia ni mafutaga vavalalata ma tumau ma so'o se tagata fa'atau i totonu o vaega e feso'ota'i.
Saunia e Alexandra mai Algeria - 2017.06.22 12:49 I se tulaga lautele, ua matou faamalieina i itu uma, taugofie, maualuga le tulaga lelei, vave ona tuuina atu ma le faiga lelei o oloa, o le a matou galulue faatasi e sosoo ai!
Saunia e Louise mai Florence - 2017.08.21 14:13 Vaega o oloa
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