Fa'atasiga Fa'ama'i Manava
Igoa o oloa
HWTS-RT050-Ituaiga E ono o Pusa Su'esu'ega Su'esu'ega Su'i Fa'ama'i(Fluorescence PCR)
Epidemiology
O le fulū, e masani ona ta'ua o le 'flu', ose fa'ama'i fa'ama'i pipisi e mafua mai i le virusi o le fulū, lea e tele fa'ama'i ma e tele lava ina pipisi e ala i le tale ma le mafatua.
Respiratory syncytial virus (RSV) o se siama RNA, e patino i le aiga paramyxoviridae.
O le Human Adenovirus (HAdV) ose fa'ama'i DNA fa'alua fa'asala e aunoa ma se teutusi.Le itiiti ifo i le 90 genotypes ua maua, lea e mafai ona vaevaeina i le 7 subgenera AG.
Human rhinovirus (HRV) o se tasi o le aiga Picornaviridae ma le Enterovirus genus.
Mycoplasma pneumoniae (MP) o se microorganism pathogenic e i le va o siama ma siama i le lapopoa.
Auala
| Auala | PCR-Mix A | PCR-Mix B |
| FAM Channel | IFV A | HAdV |
| VIC/HEX Auala | HRV | IFV B |
| CY5 Alavai | RSV | MP |
| Auala ROX | Pulea i totonu | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | -18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab |
| Ct | ≤35 |
| LoD | 500Kopi/mL |
| Fa'apitoa | 1.O fa'ai'uga o su'ega fa'afeagai na fa'aalia ai e leai se tali fa'asaga i le va o le pusa ma le coronavirus tagata SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, Parainfluenza virus type 1, 2, ma le 3, Chlamydia pneumoniae, human metapneumovirus, Enterovirus A, B, C, D, Epstein-Barr virus, Misela, cytomegalovirus tagata, Rotavirus, Norovirus, Mumps virus, Varicella-zoster virus, Legionella, Bordetella pertussis, Haemophilus influenzae, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Klebsiella pneumoniae, Mycobacterium tuberculosis, Aspergillus fumigatus, Candida albicans, Candida glabrata, Pneumocystis jiroveci, Cryptococcus neoformans ma tagata genomic nucleic acids. 2.Malosiaga fa'alavelave fa'alavelave: Mucin (60mg/mL), 10% (v/v) toto o le tagata, phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (ma fa'asao) (20mg/mL), beclomethasone ( 20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), Fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha-interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), cefprozil (40μg/mL), Meropenem (200mg/mL), levofloxacin (10μg/mL), ma le tobramycin (0.6mg/mL) na filifilia mo le suʻega faʻalavelave, ma o faʻaiʻuga na faʻaalia ai o mea faʻalavelave i faʻatonuga o loʻo i luga e leai se faʻalavelave faʻalavelave i faʻaiʻuga o suʻega o pathogens. |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Polokalama PCR Taimi Moni, BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Aofa'iga PCR Solution
