Orientia tsutsugamushi
Igoa o oloa
HWTS-OT002B Orientia tsutsugamushiPusa Su'esu'ega Nucleic Acid (Fluorescence PCR)
Epidemiology
Scrub typhus ose fa'ama'i fiva tele e mafua mai ile fa'ama'i pipisi ole Orientia tsutsugamushi (Ot). O le Orientia scrub typhus ose microorganism parasitic intracellular obligate Gram-negative. O le Orientia scrub typhus e aofia i le ituaiga Orientia i le faasologa o Rickettsiales, aiga Rickettsiaceae, ma le genus Orientia. E masani ona feavea'i le scrub typhus e ala ile u ole chigger larvae o lo'o feavea'i fa'ama'i. E faʻaalia ile falemaʻi ile fiva faʻafuaseʻi faʻafuaseʻi, eschar, lymphadenopathy, hepatosplenomegaly, ma leukopenia toto lautele, ma isi. I tulaga ogaoga, e mafai ona mafua ai le maningitis, ate ma fatugaʻo faaletonu, faʻaletonu tele-organ, ma e oʻo lava i le oti.
Auala
| FAM | Orientia tsutsugamushi |
| ROX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | sua fou |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/μL |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Polokalama PCR Taimi Moni, BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Galulue Galuega
Fautuaina le su'eina o mea fa'asusu: Macro & Micro-Su'egalauteleDNA/RNA Kit (HWTS-3019) (lea e mafai ona faʻaaogaina ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faʻatautaia le faʻaaogaina e tusa ai ma le faʻatonuga mo le faʻaogaina o lenei mea faʻapipiʻi. O le voluma fa'ata'ita'iga ua maua mai o le 200µL, ma o le voluma fa'amasani fautuaina o le100µL.
