Middle East Respiratory Syndrome Coronavirus Nucleic Acid
Igoa o oloa
HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)
Epidemiology
Middle East respiratory syndrome coronavirus (MERS-CoV), o se β-coronavirus e mafua aifaʻamaʻi manava i totonu o tagata, na muaʻi iloa i le 60-tausaga le matua Saudi Arabian male maʻi i le 24th July, 2012. O le faʻataʻitaʻiga falemaʻi o faʻamaʻi pipisi MERS-CoV e afua mai i le asymptomatic state poʻo le manava manava faʻamaʻi i le ogaoga o le manava manava e oʻo lava i le oti.
Auala
FAM | MERS virus RNA |
VIC(HEX) | Pulea i totonu |
Vaega Fa'atekinisi
Teuina | ≤-18℃ I le pogisa |
Fa'asa-ola | 9 masina |
Ituaiga Fa'ata'ita'iga | Fufulu nasopharyngeal faatoa aoina |
CV | ≤5.0% |
Ct | ≤38 |
LoD | 1000 Kopi/mL |
Fa'apitoa | E leai se feso'ota'iga fa'atasi ma tagata coronaviruses tagata coronavirus SARSr-CoV ma isi fa'ama'i masani. |
Meafaigaluega talafeagai: | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fautuaina le fa'aaogaina o le fa'aputuga: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R) e Tiangen Biotech (Beijing) Co., Ltd.
Filifiliga 2.
Fautuaina le fa'aaogaina o le fa'aputuga: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).