Fa'ama'i o le Manava i Sasa'e Tutotonu Coronavirus Nucleic Acid

Fa'amatalaga Pupuu:

O le pusa meafaigaluega e faʻaaogaina mo le iloa lelei o le MERS coronavirus nucleic acid i totonu o suʻega o le nasopharyngeal ma le Middle East Respiratory Syndrome (MERS) coronavirus.

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Fa'amatalaga o le oloa

Fa'ailoga o Oloa

Igoa o le oloa

HWTS-RT031A-Meafaigaluega mo le Su'esu'eina o le Coronavirus Nucleic Acid mo le Fa'ama'i o le Manava i Sasa'e Tutotonu (Fluorescent PCR)

Fa'ama'i pipisi

O le Middle East respiratory syndrome coronavirus (MERS-CoV), o se β-coronavirus e mafua aiO faʻamaʻi manava i tagata, na muai faʻailoaina i se tamaʻitaʻi Saudi Arabia e 60 tausaga le matua ua maliu i le aso 24 o Iulai, 2012. O le faʻaaliga faafomaʻi o le faʻamaʻi MERS-CoV e amata mai i le leai o ni faʻailoga poʻo ni faʻailoga mama o le manava i le ogaoga o faʻamaʻi manava e oʻo lava i le oti.

Alavai

FAM	RNA o le siama MERS
VIC(HEX)	Pulega i Totonu

Fa'atulagaga Fa'apitoa

Teuina	≤-18 ℃ I le pogisa
Aso e teu ai	9 masina
Ituaiga Fa'ata'ita'iga	O su'isu'i nasopharyngeal fou na aoina
CV	≤5.0%
Ct	≤38
LoD	1000 Kopi/mL
Fa'apitoa	E leai se fesoota'iga fa'atasi ma le coronavirus o tagata, le SARSr-CoV, ma isi siama fa'ama'i masani.
Meafaigaluega Fa'aoga:	Fa'aogaina o Biosystems 7500 Faiga PCR Taimi Moni Fa'aogaina o Biosystems 7500 Faiga PCR Vave i le Taimi Moni Faiga PCR Taimi Moni a le QuantStudio®5 SLAN-96P Polokalame PCR Taimi Moni Faiga PCR Taimi Moni a le LightCycler®480 Polokalama Iloa PCR Taimi Moni a le LineGene 9600 Plus MA-6000 Fa'ata'amilosaga Fa'avevela Fa'atatau i le Taimi Moni BioRad CFX96 Polokalame PCR Taimi Moni BioRad CFX Opus 96 Polokalame PCR Taimi Moni

Tafega o Galuega

Filifiliga 1.

Mea fa'aola e fautuaina mo le fa'aosoina: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R) e le Tiangen Biotech (Beijing) Co., Ltd.

Filifiliga 2.

O le vaila'au e fa'aaogā e aveese ai le vaila'au fautuaina: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

Muamua: SARS-CoV-2 Nucleic Acid

Sosoo ai: Antibody o le SARS-CoV-2 IgM/IgG

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