Klebsiella Pneumoniae, Acinetobacter Baumannii ma Pseudomonas Aeruginosa ma Vailaau Fa'asagaga Fa'asaina (KPC, NDM, OXA48 ma IMP) Multiplex

Klebsiella Pneumoniae, Acinetobacter Baumannii ma Pseudomonas Aeruginosa ma Vailaau Faʻasagaga Faʻatau (KPC, NDM, OXA48 ma IMP) Teleplex Featured Image

Fa'amatalaga Puupuu:

O lenei pusa o loʻo faʻaaogaina mo in vitro qualitative detection of Klebsiella pneumoniae (KPN), Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) ma fa carbapenem resistance genes (lea e aofia ai KPC, NDM, OXA48 ma IMP) i samples sputum tagata, e tuʻuina atu ai le faʻavaeina o togafitiga ma vailaʻau faʻamaʻi o siama faʻamaʻi.

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Fa'amatalaga Oloa

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Igoa o oloa

HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii ma Pseudomonas Aeruginosa ma Vailaau Fa'asagaga Fa'asaina (KPC, NDM, OXA48 ma IMP) Pusa Su'esu'ega Tele (Fluorescence PCR)

Tusi Faamaonia

Epidemiology

Klebsiella pneumoniae ose fa'ama'i fa'ama'i masani ma o se tasi o siama fa'ama'i taua e mafua ai fa'ama'i pipisi. A faʻaitiitia le malosi o le tino, e ulu atu le siama i totonu o mama mai le manava, ma mafua ai le faʻamaʻi i le tele o vaega o le tino, ma o le vave faʻaaogaina o vailaau faʻamaʻi o le ki lea e togafitia ai.^[1].

Ole nofoaga taatele ole fa'ama'i pipisi ole Acinetobacter baumannii ole mama, ole ma'i taua mo le niumonia maua ile Falema'i (HAP), ae maise le niumonia e feso'ota'i ai le Ventilator (VAP). E masani ona faʻatasi ma isi faʻamaʻi pipisi ma faʻamaʻi pipisi, faʻatasi ai ma uiga o le maualuga o le maʻi ma le maualuga o le oti.

Pseudomonas aeruginosa o le sili ona taatele e le faʻafefeteina gram-negative bacilli i falemaʻi faʻataʻitaʻiga, ma o se faʻamaʻi taua tele mo faʻamaʻi pipisi i le falemaʻi, faʻatasi ai ma uiga o le nofoia faigofie, faigofie fesuiaiga ma le tele o fualaau faasaina.

Auala

Igoa	PCR-Faafefiloi 1	PCR-Mix 2
FAM Channel	Aba	IMP
VIC/HEX Auala	Pulea i totonu	KPC
CY5 Alavai	PA	NDM
Auala ROX	KPN	OXA48

Vaega Fa'ainisinia

Teuina	≤-18℃
Fa'asa-ola	12 masina
Ituaiga Fa'ata'ita'iga	Sputum
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Fa'apitoa	a) O le suʻega faʻafefeteina o loʻo faʻaalia ai o lenei pusa e leai se faʻalavelave faʻafefe ma isi faʻamaʻi manava, e pei ole Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella Escherichudomona fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus ma sputum samples e aunoa ma ni sini, ma isi. b) Faʻalavelave faʻalavelave: Filifili mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, ma roxithromycin, ma isi mo le suʻega faʻalavelave, ma o faʻaiʻuga o loʻo faʻaalia ai o mea faʻalavelave faʻalavelave o loʻo taʻua i luga e le mafai ona maua le niumonia. Acinetobacter baumannii, Pseudomonas aeruginosa ma carbapenem teteʻe genes KPC, NDM, OXA48 ma IMP.
Meafaigaluega Faatatau	Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio^®5 Faiga PCR Taimi Moni LightCycler^®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Bioer technology) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System