Fulu A/B
Igoa o oloa
HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Ole fa'ama'i ole Influenza A ose fa'ama'i fa'ama'i fa'ama'i fa'ama'i, ma le tele o vaega laiti e pei ole H1N1 ma le H3N2, e faigofie ona suia ma sosolo i le lalolagi atoa.O le suiga o le antigenic e faasino i le suiga o le siama o le influenza A, e mafua ai le tulaʻi mai o se subtype fou.O fa'ama'i fa'ama'i fa'ama'i B ua vaevaeina i ni gafa tetele se lua, o Yamagata ma Vitoria.O fa'ama'i fa'ama'i ole Influenza B e na'o le antigenic drift, ma e 'alo'ese mai le mata'ituina ma le fa'amamaina ole puipuiga ole tagata e ala ia latou suiga.Ae ui i lea, o le fua faatatau o le evolusione o le influenza B virus e telegese nai lo le influenza A virus, ma o le influenza B virus e mafai foi ona mafua ai le fa'ama'i o le manava o le tagata ma o'o atu ai i fa'ama'i.
Auala
| FAM | IFV A |
| ROX | Pulea i totonu |
| VIC/HEX | IFV B |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | oropharyngeal swab |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A:500Kopi/mL,IFV B:500Kopi/mL |
| Fa'apitoa | . enterovirus, parainfluenza virus, misle virus, human metapneumovirus, mumps virus, respiratory syncytial virus type B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, moraxella catarrhalis mycobacteria, myoplasma catarrhalis , neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius ma DNA genomic tagata. 2. Suʻega faʻalavelave: O mea faʻalavelave e filifilia e pei o le mucin (60mg/mL), toto o le tagata, oxymetazoline (2mg/mL), sulfur (10%), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL). ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), ma fa'ai'uga fa'aalia. o mea fa'alavelave fa'atasi ma fa'atonuga o lo'o i luga e leai se fa'alavelave fa'alavelave i le mauaina o le pusa. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Polokalama PCR Taimi Moni ma BioRad CFX Opus 96 Faiga PCR Taimi Moni |
Galulue Galuega
Filifiliga 1.
Reagents fa'atonuina mo le fa'aaogaina: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona fa'aoga i Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faia le fa'aputuga e tusa ai ma le IFU.Ole fua ole fa'ata'ita'iga ole200μL.Ole fua ole elution fautuaina ole 80μL.
Filifiliga 2.
Fa'atonuga fa'afo'i fa'aputuga: Macro & Micro-Su'ega Fa'ata'ita'i Fa'ata'ita'i Fa'asa'o (HWTS-3005-8).E tatau ona faia le su'ega e tusa ai ma le IFU.
Filifiliga 3.
Fautuaina le fa'aaogaina o mea e fa'aaogaina: Nucleic Acid Extraction po'o le Pusa Fa'amamāina (YDP315-R).E tatau ona faia le su'ega e tusa ai ma le IFU.Ole faʻataʻitaʻiga faʻataʻitaʻiga voluma ole 140μL.Ole fua ole elution fautuaina ole 60μL.
