Fufula A Virus Universal/H1/H3
Igoa o oloa
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
O le fa'ama'i fa'ama'i ose ituaiga sui o Orthomyxoviridae. O se fa'ama'i e matua'i lamatia ai le soifua maloloina o tagata. E mafai ona afaina tele ai le tagata talimalo. O le fa'ama'i fa'avaitaimi e a'afia ai le tusa ma le 600 miliona tagata i le lalolagi atoa ma mafua ai le 250,000 ~ 500,000 maliu, o le influenza A virus o le mafua'aga autu lea o fa'ama'i ma le oti. O le fa'ama'i o le Influenza A o se RNA fa'aleaga e tasi le la'au. E tusa ai ma ona luga hemagglutinin (HA) ma neuraminidase (NA), HA e mafai ona vaevaeina i 16 subtypes, NA Vaevae i 9 subtypes. I totonu o fa'ama'i fa'ama'i A, o vaega laiti o fa'ama'i fulū e mafai ona a'afia sa'o ai tagata: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 ma H10N8. Faatasi ai ma i latou, H1 ma H3 subtypes e sili ona faʻamaʻi, ma e sili ona aoga le gauai.
Auala
| FAM | influenza O se fa'ama'i fa'ama'i fa'ama'i lautele |
| VIC/HEX | influenza A H1 ituaiga virus nucleic acid |
| ROX | influenza A H3 ituaiga virus nucleic acid |
| CY5 | pulea totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | su'ega nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/μL |
| Fa'apitoa | Leai se fa'afeagai fa'atasi ma isi fa'ata'ita'iga o le manava e pei ole Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumo/B1/B2, Coronavirus A1/B2, Virus A1/B2, Coronavirus. 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, ma isi ma DNA genomic tagata. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R) e Tiangen Biotech (Beijing) Co.,Ltd. E tatau ona faʻatinoina le faʻaogaina e tusa ai ma faʻatonuga mo le faʻaaogaina. O le voluma faʻataʻitaʻiga na maua mai o le 140μL, ma o le voluma elution fautuaina o le 60μL.
Filifiliga 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). E tatau ona faʻatinoina le faʻaogaina e tusa ai ma faʻatonuga mo le faʻaaogaina. O le voluma fa'ata'ita'iga na maua mai o le 200μL, ma o le voluma elution fautuaina o le 80μL.
