Fufula A Virus Universal/H1/H3
Igoa o oloa
HWTS-RT012 Fufula A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
Mycoplasma pneumoniae (MP) ole la'ititi la'ititi la'ititi ole prokaryotic ma se fausaga sela ma leai se puipui i le va o siama ma siama.O le MP e mafua ai faʻamaʻi pipisi o le manava i tagata, aemaise lava i tamaiti ma tupulaga talavou.MP e mafai ona mafua ai le Mycoplasma hominis pneumonia, faʻamaʻi pipisi o le manava i tamaiti ma le niumonia atypical.E eseese auga o le falema'i, tele ogaoga tale, fiva, ma'alili, ulu tiga, tiga fa'a'i, fa'ama'i pito i luga o le manava ma le bronchopneumonia e sili ona taatele.O nisi o gasegase e ono maua i le niumonia tuga mai le fa'ama'i pito i luga o le manava, ma e ono tupu ai le tiga tele o le manava po'o le oti.O le MP o se tasi o faʻamaʻi masani ma taua i le niumonia e maua e le nuʻu (CAP), faʻamauina mo le 10% -30% o le CAP, ma e mafai ona faʻateleina le 3-5 taimi pe a faʻateleina le MP.I tausaga talu ai nei, o le aofaʻi o MP i CAP pathogens ua faasolosolo malie ona faʻateleina.O le aʻafiaga o le Mycoplasma pneumoniae ua faʻateleina, ma ona o ona faʻaaliga faʻapitoa faʻapitoa, e faigofie ona fenumiai ma siama siama ma viral malulu.O le mea lea, o le vave su'esu'eina o fale su'esu'e e taua tele mo su'esu'ega ma togafitiga.
Auala
| FAM | influenza O se fa'ama'i fa'ama'i fa'ama'i lautele |
| VIC/HEX | influenza A H1 ituaiga virus nucleic acid |
| ROX | influenza A H3 ituaiga virus nucleic acid |
| CY5 | pulea totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | su'ega nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/μL |
| Fa'apitoa | Leai se feso'ota'iga fa'atasi ma isi fa'ata'ita'iga o le manava e pei ole Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3 , Coxsackie virus, Echo virus, Metapneumovirus A1/A2 B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, ma isi ma DNA genomic tagata. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R) e Tiangen Biotech (Beijing) Co., Ltd.E tatau ona fa'atinoina le fa'atosina e tusa ai ma fa'atonuga mo le fa'aoga.O le voluma faʻataʻitaʻiga na maua mai o le 140μL, ma o le voluma elution fautuaina o le 60μL.
Filifiliga 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).E tatau ona fa'atinoina le fa'atosina e tusa ai ma fa'atonuga mo le fa'aoga.O le voluma faʻataʻitaʻiga na maua mai o le 200μL, ma le faʻataʻitaʻiga o le elution voluma e 80μL.
