Tagata PML-RARA Fusion Gene Mutation
Igoa o oloa
HWTS-TM017ATagata PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
Acute promyelocytic leukemia (APL) ose ituaiga fa'apitoa ole ma'i leukemia myeloid (AML). E tusa ma le 95% o tagata maʻi APL o loʻo faʻatasi ma se suiga faʻapitoa cytogenetic, e taʻua o t(15;17)(q22;q21), lea e faʻapipiʻiina ai le gene PML i le chromosome 15 ma le retinoic acid receptor α gene (RARA) i luga o le chromosome 17 faʻapipiʻiina e fausia ai le PML-RARA fusion gene. Ona o motuga eseese o le gene PML, o le PML-RARA fusion gene e mafai ona vaevaeina i ituaiga umi (ituaiga L), ituaiga pupuu (ituaiga S) ma ituaiga eseese (ituaiga V), e tusa ma le 55%, 40% ma le 5% i le faasologa.
Auala
| FAM | PML-RARA fusion gene |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ I le pogisa |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | ga'o ponaivi |
| CV | <5.0％ |
| LoD | 1000 Kopi/mL. |
| Fa'apitoa | E leai se feso'ota'iga fa'afeagai ma isi genes fusuaga BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, ma TEL-AML1 fusion genes |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Fa'atonu PCR Taimi Moni (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Fa'atonuga fa'a'ese'esega: RNAprep Toto Ma'ema'e Aofa'i RNA Pusa Ave'esega (DP433). E tatau ona faia le su'ega e tusa ai ma le IFU.
