Afete E Virus
Igoa o oloa
HWTS-HP006 Virus Hepatitis E Su'esu'eina Asi Nucleic Kit (Fluorescence PCR)
Epidemiology
Fa'ama'i Hepatitis E (HEV) ose virusi RNA e mafua ai fa'afitauli o le soifua maloloina o le lalolagi. O lo'o i ai le lautele o le 'au talimalo ma ei ai le meatotino o le sopoia o pa puipui interspecies. O se tasi lea o fa'ama'i fa'ama'i e sili ona taua ma mafua ai le afaina tele i tagata ma manu. O le HEV e masani lava ona feavea'i e ala ile fecal-oral transmission, ma e mafai fo'i ona feavea'i tu'u sa'o ile embryos po'o le toto. Faatasi ai ma i latou, i le fecal-oral auala feʻaveaʻi, vai faʻaleagaina HEV ma meaʻai e salalau lautele, ma o le lamatiaga o faʻamaʻi HEV i tagata ma manu e maualuga [1-2].
Auala
| FAM | HEV nucleic acid |
| ROX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Usu fa'a'i |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/μL |
| Fa'apitoa | Fa'ama'i Hepatitis E (HEV) ose virusi RNA e mafua ai fa'afitauli o le soifua maloloina o le lalolagi. O lo'o i ai le lautele o le 'au talimalo ma ei ai le meatotino o le sopoia o pa puipui interspecies. O se tasi lea o fa'ama'i fa'ama'i e sili ona taua ma mafua ai le afaina tele i tagata ma manu. O le HEV e masani lava ona feavea'i e ala ile fecal-oral transmission, ma e mafai fo'i ona feavea'i tu'u sa'o ile embryos po'o le toto. Faatasi ai ma i latou, i le fecal-oral auala feʻaveaʻi, vai faʻaleagaina HEV ma meaʻai e salalau lautele, ma o le lamatiaga o faʻamaʻi HEV i tagata ma manu e maualuga [1-2]. |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni (FQD-96A, Hangzhou Bioer tekinolosi) MA-6000 Taimi Moni Fua Fa'ata'avale vevela (Suzhou Molarray Co., Ltd.) BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ma Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006C). E tatau ona aveese mai e tusa ai ma faatonuga. Ole voluma fa'amama fa'atonuina ole 80µL.
Filifiliga 2
TIANamp Virus DNA/RNA Kit (YDP315-R) gaosia e Tiangen Biotech (Beijing) Co., Ltd. E tatau ona aveese faʻapitoa e tusa ai ma faatonuga. O le voluma fa'ata'ita'iga na maua mai o le 140μL. O le voluma elution fautuaina e 60µL.v
