HCV Genotyping
Igoa Oloa
HWTS-HP004-HCV Pusa Su'esu'ega Genotyping (Fluorescence PCR)
Epidemiology
Hepatitis C virus (HCV) e patino i le aiga flaviviridae, ma o lona genome o se RNA lelei e tasi, lea e faigofie ona suia.Ole siama e iai ile hepatocytes, serum leukocytes ma le plasma o tagata ua aafia.O kenera HCV e faigofie ona suia ma e mafai ona vaevaeina i le itiiti ifo ma le 6 genotypes ma le tele o subtypes.'ese'ese HCV genotypes 'ese'ese le fa'aogaina ole DAA ole togafitiga ma kosi o togafitiga.O le mea lea, aʻo leʻi togafitia tagata mamaʻi i le DAA antiviral therapy, e tatau ona iloa le genotype HCV, ma e oʻo lava i tagata mamaʻi i le ituaiga 1, e tatau ona iloa pe o le ituaiga 1a poʻo le ituaiga 1b.
Auala
| FAM | Ituaiga 1b, Ituaiga 2a |
| ROX | Ituaiga 6a, Ituaiga 3a |
| VIC/HEX | Pulea totonu, Ituaiga 3b |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ I le pogisa |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Serum, Plasma |
| Ct | ≤36 |
| CV | ≤5.0％ |
| LoD | 200 IU/mL |
| Fa'apitoa | Fa'aoga le pusa lea e su'e ai isi fa'ama'i po'o siama fa'ata'ita'iga pei ole: human cytomegalovirus, Epstein-Barr virus, human immunodeficiency virus, hepatitis B virus, hepatitis A virus, syphilis, human herpes virus type 6, herpes simplex virus type 1, simplex Herpes virus ituaiga 2, influenza A virus, Propionibacterium acnes, Staphylococcus aureus, Candida albicans, ma isi. O taunuuga e le lelei uma. |
| Meafaigaluega Faatatau | E mafai ona fetaui ma meafaifa'aili PCR fa'aulu i luga o le maketi. ABI 7500 Faiga PCR Taimi Moni ABI 7500 Fa'atonuga PCR Taimi Moni Anapogi SLAN-96P Faiga PCR Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
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