Hantaan Virus Nucleic
Igoa o oloa
HWTS-FE005 Hantaan Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Hantavirus o se ituaiga o atigipusa, vaelua, leaga-strand RNA virus. Ua vaevaeina le Hantavirus i ituaiga e lua: o le tasi e mafua ai le Hantavirus pulmonary syndrome (HPS), ma le isi e mafua ai le Hantavirus hemorrhagic fever with renal syndrome (HFRS). O le mea muamua e masani ona taatele i Europa ma le Iunaite Setete, ma o le mea mulimuli o le fiva toto ma le ma'i fatu e mafua mai i le Hantaan virus, lea e taatele i Saina. O fa'ailoga o le hantavirus type e masani ona fa'aalia o le fiva toto ma le ma'i fatu, lea e fa'aalia i le maualuga o le fiva, toto maualuga, toto, oliguria po'o le polyuria ma isi fa'aletonu o le tino. E fa'ama'i i tagata ma e tatau ona lava le fa'alogo i ai.
Auala
| FAM | ituaiga hantavirus |
| ROX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | sua fou |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Kopi/μL |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Reagents fa'atonuina: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (lea e mafai ona fa'aogaina ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faia le fa'aputuga e tusa ai ma le IFU. Ole faʻataʻitaʻiga faʻataʻitaʻiga voluma ole 200μL. Ole fua ole elution fautuaina ole 80μL.
Fautuaina le fa'aaogaina o mea e fa'aaogaina: Nucleic Acid Extraction po'o le Pusa Fa'amamāina (YDP315-R). E tatau ona faia le su'ega e tusa ai ma le IFU. Ole faʻataʻitaʻiga faʻataʻitaʻiga voluma ole 140μL. Ole fua ole elution fautuaina ole 60μL.
