Enterovirus Universal, EV71 ma CoxA16
Igoa Oloa
HWTS-EV026B-Enterovirus Universal, EV71 ma CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
HWTS-EV020Y/Z-Aisa-mamago Enterovirus Universal, EV71 ma CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
Tusi Faamaonia
CE/MDA(HWTS-EV026)
Epidemiology
O fa'ama'i lima vae-gutu (HFMD) ose fa'ama'i fa'ama'i fa'ama'i tele i tamaiti. E tele lava ina tupu i tamaiti i lalo ifo o le 5 tausaga, ma e mafai ona mafua ai le herpes i lima, vae, gutu ma isi vaega, ma o se vaega toalaiti o tamaiti e mafai ona mafua ai faʻalavelave e pei o le myocarditis, pulmonary edema, aseptic maningoencephalitis, ma isi.
I le taimi nei, 108 serotypes o enteroviruses ua maua, lea ua vaevaeina i ni vaega se fa: A, B, C ma D. Enteroviruses e mafua ai le HFMD eseese, ae enterovirus 71 (EV71) ma coxsackievirus A16 (CoxA16) e sili ona taatele ma faaopoopo atu i le HFMD, e mafai ona mafua ai le ogaoga o le maʻi fatu fatugalemu complications, maʻi pipili, maʻi pipili.
Auala
| FAM | Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Pulea totonu |
Vaega Fa'ainisinia
| Teuina | Suavai: ≤-18℃ I le pogisaLyophilization: ≤30 ℃ |
| Fa'asa-ola | Suavai: 9 masinaLyophilization: 12 masina |
| Ituaiga Fa'ata'ita'iga | Fa'ata'ita'iga o le fa'a'i, sua o le Herpes |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500Kopi/mL |
| Meafaigaluega Faatatau | E mafai ona fetaui ma meafaifa'aili PCR fa'aulu i luga o le maketi.ABI 7500 Faiga PCR Taimi Moni ABI 7500 Fa'atonuga PCR Taimi Moni Anapogi SLAN-96P Faiga PCR Taimi Moni QuantStudio®5 Faiga PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Aofa'iga PCR Solution
Fautuaina le faʻaaogaina o le faʻaaogaina: Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (lea e mafai ona faʻaaogaina ma le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona faia le faʻatonuga e tusa ai ma le tusi lesona. O le voluma fa'ata'ita'iga na maua mai o le 200μL, ma o le voluma elution fautuaina o le 80μL.
Fautuaina le fa'asusuina o mea fa'apipi'i: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8). E tatau ona faia le su'ega e tusa ai ma le tusi lesona. O fa'ata'ita'iga o fa'ata'ita'iga o fa'ata'ita'iga o su'a ole oropharyngeal po'o fa'ata'ita'iga ole sua ole herpes o gasegase na aoina ile nofoaga. Fa'apipi'i sa'o le swabs ua aoina i le Macro & Micro-Test Sample Release Reagent, vortex ma fa'afefiloi lelei, tu'u i le vevela o le potu mo le 5 minute, ave i fafo ona fesuia'i lea ma fa'afefiloi lelei ina ia maua le RNA o fa'ata'ita'iga ta'itasi.
Fautuaina le fa'aaogaina o le fa'aputuga: QIAamp Viral RNA Mini Kit (52904) e le QIAGEN po'o le Nucleic Acid Extraction po'o le Purification Reagent (YDP315-R). E tatau ona fa'ataunu'uina le fa'aputuga e tusa ma le tusi fa'atonuga.
