Enterovirus 71 (EV71)
Igoa o oloa
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
O fa'ama'i lima-vae-gutu ose fa'ama'i pipisi e mafua mai ile enteroviruses (EV). I le taimi nei, 108 ituaiga o serotypes o enteroviruses ua maua, lea e vaevaeina i ni vaega se fa: A, B, C ma D. Faatasi ai ma i latou, enterovirus EV71 ma CoxA16 o pathogens autu. Ole fa'ama'i e tele lava ina tupu i tamaiti i lalo ole 5 tausaga, ma e mafai ona mafua ai le herpes i lima, vae, gutu ma isi vaega. O se numera to'aitiiti o tamaiti o le a atia'e fa'alavelave e pei ole myocarditis, pulmonary edema, ma aseptic maningoencephalitis.
Auala
| FAM | EV71 |
| ROX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 9 masina |
| Ituaiga Fa'ata'ita'iga | Oropharyngeal swab,Herpes vai |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500Kopi/mL |
| Meafaigaluega Faatatau | Fa'aogaina Biosystems 7500 Fa'atonu PCR Taimi Moni Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Faiga PCR Taimi Moni LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
Fautuaina le faʻaaogaina o le faʻaaogaina: Macro & Micro-Test Sample Reagent Reagent (HWTS-3005-8), ma e tatau ona faia le faʻaogaina e tusa ai ma faatonuga mo le faʻaaogaina. O fa'ata'ita'iga na maua mai o su'ega ole oropharyngeal po'o fa'ata'ita'iga ole sua ole herpes mai gasegase na aoina i luga ole nofoaga. Fa'apipi'i sa'o le swabs ua aoina i le Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), vortex ma fa'afefiloi lelei, tu'u i le vevela o le potu mo le 5 minute, ave i fafo ona fesuia'i lea e fa'afefiloi lelei, e maua ai le RNA o fa'ata'ita'iga ta'itasi.
Filifiliga 2.
Reagent fa'asaoina fautuaina: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor-30B03) saunia e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ma e tatau ona faia le faʻaaogaina e tusa ai ma faatonuga mo le faʻaaogaina. O le voluma fa'ata'ita'iga na maua mai o le 200µL, ma o le voluma fa'ata'ita'i fautuaina o le 80μL.
Filifiliga3.
Fautuaina le fa'aaogaina o le fa'aputuga: QIAamp Viral RNA Mini Kit (52904) e le QIAGEN po'o le TIANamp Virus DNA/RNA Kit (YDP315-R), ma e tatau ona fa'atinoina le fa'agasologa e tusa ai ma le fa'atonuga mo le fa'aoga. O le voluma fa'ata'ita'iga na maua mai o le 140μL, ma o le voluma elution fautuaina o le 60μL.
