Coxsackie Virus Type A16 Nucleic Acid

O lenei pusa o loʻo faʻaaogaina le Enzymatic Probe Isothermal Amplification (EPIA), ma mamanuina faʻamatalaga faʻapitoa ma suʻega faʻavae RNA (rProbe) mo le itulagi sili ona faʻasaoina o Cox A16, ma faʻaopoopo le Bst enzyme ma le RNaseH enzyme i le taimi e tasi, lea o le agavale ma pito taumatau o le RNA faavae o le rProbe o loʻo faʻailogaina i vaega faʻafefe ma tineia, taʻitasi.Fa'aaoga le DNA polymerase activity ma le strand displacement activity of Bst enzyme e fa'alautele ai le sini e su'eina i se vevela faifaipea, e mafai e le enzyme RNaseH ona fa'amavaeina le RNA fa'avae i luga o le filifili fa'atosina-su'esu'e, ina ia mafai ai e le kulupu felanulanua'i ma tineia o rProbe ona tuueseeseina lea o le fluorescing.E le gata i lea, o le vaega agavale o le vaega totoe rProbe RNA e mafai ona faʻaaogaina e fai ma faʻamuamua e faʻalautele atili e fausia ai se oloa, lea e faʻaputu atili ai le oloa.O le faailo fluorescent o loʻo faʻaauau pea ona faʻaputuina i le faʻavaeina o le oloa, ma iloa ai le suʻesuʻeina o le acid nucleic target.