Borrelia Burgdorferi Nucleic Acid
Igoa o oloa
HWTS-OT068 Borrelia Burgdorferi Nucleic Acid Detection Kit(Fluorescence PCR)
Tusi Faamaonia
CE
Epidemiology
O le fa'ama'i Lyme e mafua mai i fa'ama'i pipisi mai le Borrelia burgdorferi ma e tele lava ina fe'avea'i i le va o manu, i le va o manu fa'afeiloa'i ma tagata e ala i siaki malo.O le pathogen Borrelia burgdorferi e mafai ona mafua ai le Erythema chronicum migrans tagata, faʻapea foʻi ma faʻamaʻi e aofia ai le tele o faiga e pei o le fatu, neura, ma sooga, ma isi, ma faʻaaliga faʻapitoa e eseese.E tusa ai ma le atinaʻeina o faʻamaʻi faʻamaʻi, e mafai ona vaevaeina i faʻamaʻi faʻamaʻi vave, faʻamaʻi pipisi faʻasalalau ma faʻamaʻi pipisi tuai, lea e afaina tele ai le soifua maloloina o tagata.O le mea lea, i le suʻesuʻega faʻapitoa o Borrelia burgdorferi, e taua tele le faʻavaeina o se auala faigofie, faʻapitoa ma vave mo suʻesuʻega etiological o Borrelia burgdorferi.
Auala
| FAM | DNA o Borrelia burgdorferi |
| VIC/HEX | Pulea i totonu |
Vaega Fa'atekinisi
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | Fa'ata'ita'iga toto atoa |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kopi/mL |
| Meafaigaluega Faatatau | ABI 7500 Faiga PCR Taimi Moni ABI 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fa'ata'amiloga Fa'avevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Filifiliga 1.
QIAamp DNA Blood Midi Kit e Qiagen (51185).It e tatau ona aumaie tusa ai ma le atoatoai le faatonuga, ma le volume elution fautuaina e100μL.
Filifiliga 2.
TotoGDNA talavouEpusa fa'atosina (DP318,Leai.: JingchangFaamaumauga o masini20210062) gaosia e Tiangen Biochemical Technology (Beijing) Co., Ltd.. It e tatau ona aumaie tusa ai ma le atoatoai le faatonuga, ma le volume elution fautuaina e100μL.
Filifiliga 3.
Wizard® Genomic DNA Purification Kit (A1120) saunia e Promega.It e tatau ona aumaie tusa ai ma le atoatoai le faatonuga, ma le volume elution fautuaina e100μL.
