ALDH Genetic Polymorphism
Igoa o oloa
HWTS-GE015ALDH Genetic Polymorphism Detection Kit (ARMS -PCR)
Epidemiology
ALDH2 gene (acetaldehyde dehydrogenase 2), o loʻo maua i luga o le chromosome 12 o le tagata. ALDH2 o loʻo i ai le esterase, dehydrogenase ma le reductase i le taimi e tasi. O suʻesuʻega ua faʻaalia ai o le ALDH2 o se enzyme metabolic o le nitroglycerin, lea e liua ai le nitroglycerin i le nitric oxide, ma faʻamalieina ai toto toto ma faʻaleleia ai le tafe toto. Ae ui i lea, o loʻo i ai polymorphisms i le ALDH2 gene, lea e faʻapitoa i Asia i Sasaʻe. Ole ituaiga ole ALDH2 * 1/* 1 GG e malosi malosi, ae o le heterozygous e naʻo le 6% o le gaioiga o le enzyme vao, ma o le mutant homozygous e toetoe lava a leai se gaioiga o le enzyme, ma le metabolism e matua vaivai lava ma e le mafai ona ausia le manaʻoga, ma faʻaleagaina ai le tino o le tagata.
Auala
| FAM | ALDH2 |
| ROX | Pulea i totonu |
Vaega Fa'ainisinia
| Teuina | ≤-18℃ |
| Fa'asa-ola | 12 masina |
| Ituaiga Fa'ata'ita'iga | EDTA toto anticoagulated |
| CV | <5.0％ |
| LoD | 103Kopi/mL |
| Meafaigaluega Faatatau | Applied Biosystems 7500 Real-Time PCR System Fa'aogaina Biosystems 7500 Fa'atonuga PCR Taimi Moni Anapogi QuantStudio®5 Faiga PCR Taimi Moni SLAN-96P Faiga PCR Taimi Moni LightCycler®480 Taimi moni PCR faiga LineGene 9600 Plus Faiga Fa'amatalaga PCR Taimi Moni MA-6000 Taimi Moni Fua Fa'ata'avale vevela BioRad CFX96 Taimi Moni PCR System BioRad CFX Opus 96 Taimi moni PCR System |
Galulue Galuega
Fa'atonuga fa'afo'iina: fa'aoga le Blood Genome DNA Extraction Kit (DP318) e Tiangen Biotech (Beijing) Co.,Ltd. poo le Blood Genome Extraction Kit (A1120) e Promega e aveese mai ai le EDTA anticoagulated toto Genomic DNA.
Reagents fa'atonuina: Macro & Micro-Test General DNA / RNA Kit (HWTS-3019) (lea e mafai ona fa'aoga i le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) e Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E tatau ona fa'atonu le fa'atonuga e tusa ai ma faatonuga. Ole voluma elution fautuaina ole100μL.
